http://purl.uniprot.org/citations/27762343 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/27762343 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/27762343 | http://www.w3.org/2000/01/rdf-schema#comment | "Protein synthesis in the chloroplast is mediated by the chloroplast ribosome (chloro-ribosome). Overall architecture of the chloro-ribosome is considerably similar to the Escherichia coli (E. coli) ribosome but certain differences are evident. The chloro-ribosome proteins are generally larger because of the presence of chloroplast-specific extensions in their N- and C-termini. The chloro-ribosome harbours six plastid-specific ribosomal proteins (PSRPs); four in the small subunit and two in the large subunit. Deletions and insertions occur throughout the rRNA sequence of the chloro-ribosome (except for the conserved peptidyl transferase center region) but the overall length of the rRNAs do not change significantly, compared to the E. coli. Although, recent advancements in cryo-electron microscopy (cryo-EM) have provided detailed high-resolution structures of ribosomes from many different sources, a high-resolution structure of the chloro-ribosome is still lacking. Here, we present a cryo-EM structure of the large subunit of the chloro-ribosome from spinach (Spinacia oleracea) at an average resolution of 3.5 Å. High-resolution map enabled us to localize and model chloro-ribosome proteins, chloroplast-specific protein extensions, two PSRPs (PSRP5 and 6) and three rRNA molecules present in the chloro-ribosome. Although comparable to E. coli, the polypeptide tunnel and the tunnel exit site show chloroplast-specific features."xsd:string |
http://purl.uniprot.org/citations/27762343 | http://purl.org/dc/terms/identifier | "doi:10.1038/srep35793"xsd:string |
http://purl.uniprot.org/citations/27762343 | http://purl.org/dc/terms/identifier | "doi:10.1038/srep35793"xsd:string |
http://purl.uniprot.org/citations/27762343 | http://purl.uniprot.org/core/author | "Yin Z."xsd:string |
http://purl.uniprot.org/citations/27762343 | http://purl.uniprot.org/core/author | "Yin Z."xsd:string |
http://purl.uniprot.org/citations/27762343 | http://purl.uniprot.org/core/author | "Ahmed T."xsd:string |
http://purl.uniprot.org/citations/27762343 | http://purl.uniprot.org/core/author | "Ahmed T."xsd:string |
http://purl.uniprot.org/citations/27762343 | http://purl.uniprot.org/core/author | "Bhushan S."xsd:string |
http://purl.uniprot.org/citations/27762343 | http://purl.uniprot.org/core/author | "Bhushan S."xsd:string |
http://purl.uniprot.org/citations/27762343 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
http://purl.uniprot.org/citations/27762343 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
http://purl.uniprot.org/citations/27762343 | http://purl.uniprot.org/core/name | "Sci. Rep."xsd:string |
http://purl.uniprot.org/citations/27762343 | http://purl.uniprot.org/core/name | "Sci. Rep."xsd:string |
http://purl.uniprot.org/citations/27762343 | http://purl.uniprot.org/core/pages | "35793"xsd:string |
http://purl.uniprot.org/citations/27762343 | http://purl.uniprot.org/core/pages | "35793"xsd:string |
http://purl.uniprot.org/citations/27762343 | http://purl.uniprot.org/core/title | "Cryo-EM structure of the large subunit of the spinach chloroplast ribosome."xsd:string |
http://purl.uniprot.org/citations/27762343 | http://purl.uniprot.org/core/title | "Cryo-EM structure of the large subunit of the spinach chloroplast ribosome."xsd:string |
http://purl.uniprot.org/citations/27762343 | http://purl.uniprot.org/core/volume | "6"xsd:string |
http://purl.uniprot.org/citations/27762343 | http://purl.uniprot.org/core/volume | "6"xsd:string |
http://purl.uniprot.org/citations/27762343 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27762343 |
http://purl.uniprot.org/citations/27762343 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27762343 |
http://purl.uniprot.org/citations/27762343 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27762343 |
http://purl.uniprot.org/citations/27762343 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27762343 |