| http://purl.uniprot.org/citations/27792783 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27792783 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27792783 | http://www.w3.org/2000/01/rdf-schema#comment | "Non-rod non-cone photopigments in the eyes and the brain can directly mediate non-visual functions of light in non-mammals. This was supported by our recent findings on vertebrate ancient long (VAL)-opsin photopigments encoded by the val-opsinA (valopa) and val-opsinB (valopb) genes in zebrafish. However, the physiological functions of valop isoforms remain unknown. Here, we generated valop-mutant zebrafish using CRISPR/Cas genome editing, and examined the phenotypes of loss-of-function mutants. F0 mosaic mutations and germline transmission were confirmed via targeted insertions and/or deletions in the valopa or valopb gene in F1 mutants. Based on in silico analysis, frameshift mutations converted VAL-opsin proteins to non-functional truncated forms with pre-mature stop codons. Most F1 eggs or embryos from F0 female valopa/b mutants showed either no or only partial chorion elevation, and the eggs or embryos died within 26 hour-post-fertilization. However, most F1 embryos from F0 male valopa mutant developed but hatched late compared to wild-type embryos, which hatched at 4 day-post-fertilization. Late-hatched F1 offspring included wild-type and mutants, indicating the parental effects of valop knockout. This study shows valop gene knockout affects chorion formation and embryonic hatching in the zebrafish."xsd:string |
| http://purl.uniprot.org/citations/27792783 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0165535"xsd:string |
| http://purl.uniprot.org/citations/27792783 | http://purl.uniprot.org/core/author | "Moriya S."xsd:string |
| http://purl.uniprot.org/citations/27792783 | http://purl.uniprot.org/core/author | "Moriya S."xsd:string |
| http://purl.uniprot.org/citations/27792783 | http://purl.uniprot.org/core/author | "Ogawa S."xsd:string |
| http://purl.uniprot.org/citations/27792783 | http://purl.uniprot.org/core/author | "Ogawa S."xsd:string |
| http://purl.uniprot.org/citations/27792783 | http://purl.uniprot.org/core/author | "Parhar I.S."xsd:string |
| http://purl.uniprot.org/citations/27792783 | http://purl.uniprot.org/core/author | "Parhar I.S."xsd:string |
| http://purl.uniprot.org/citations/27792783 | http://purl.uniprot.org/core/author | "Hang C.Y."xsd:string |
| http://purl.uniprot.org/citations/27792783 | http://purl.uniprot.org/core/author | "Hang C.Y."xsd:string |
| http://purl.uniprot.org/citations/27792783 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27792783 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27792783 | http://purl.uniprot.org/core/name | "PLoS ONE"xsd:string |
| http://purl.uniprot.org/citations/27792783 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/27792783 | http://purl.uniprot.org/core/pages | "E0165535"xsd:string |
| http://purl.uniprot.org/citations/27792783 | http://purl.uniprot.org/core/pages | "e0165535"xsd:string |
| http://purl.uniprot.org/citations/27792783 | http://purl.uniprot.org/core/title | "Deep Brain Photoreceptor (val-opsin) Gene Knockout Using CRISPR/Cas Affects Chorion Formation and Embryonic Hatching in the Zebrafish."xsd:string |
| http://purl.uniprot.org/citations/27792783 | http://purl.uniprot.org/core/title | "Deep Brain Photoreceptor (val-opsin) Gene Knockout Using CRISPR/Cas Affects Chorion Formation and Embryonic Hatching in the Zebrafish."xsd:string |
| http://purl.uniprot.org/citations/27792783 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/27792783 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/27792783 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27792783 |
| http://purl.uniprot.org/citations/27792783 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27792783 |
| http://purl.uniprot.org/citations/27792783 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27792783 |