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http://purl.uniprot.org/citations/27813598http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/27813598http://www.w3.org/2000/01/rdf-schema#comment"Helicobacter pylori employs unique methods to colonize the stomach, which induces chronic inflammation. It is also able to avoid eradication by macrophages and other immune cells. Leukocyte cell-derived chemotaxin 2 (LECT2), a multi-functional cytokine involved in many pathological conditions, has recently been shown to activate macrophages via the CD209a receptor. Therefore, we aimed to investigate the effects of LECT2 on H. pylori-infected macrophages. Macrophages were treated with recombinant LECT2, and both their ability to kill H. pylori and produce nitric oxide were analyzed. Western blot was performed to determine nuclear translocation and protein phosphorylation of p65, a subunit of nuclear factor (NF)-κB. Transfection experiments were performed to analyze the signaling pathway of LECT2 in macrophages. We found that treatment with LECT2 enhanced H. pylori killing and nitric oxide production in macrophages. In addition, DNA-binding activity and nuclear translocation of p65 were up-regulated by LECT2 treatment. Furthermore, we found that NF-κB activation by LECT2 was mediated by Raf-1 in macrophages, and Raf-1 phosphorylation was specifically altered in response to LECT2. Moreover, LECT2 induced Ser28 phosphorylation in the intracellular domain of CD209a. CD209a Ser28 phosphorylation was required for LECT2-induced Raf-1 and NF-κB activation in RAW264.7 macrophages. Our study showed that the effects of LECT2 on H. pylori killing and nitric oxide production were dependent on CD209a phosphorylation, Raf-1, and NF-κB activation. Together, these results demonstrate for the first time that exposure to LECT2 can modulate specific intracellular mechanisms downstream of CD209a to enhance H. pylori killing and nitric oxide production in macrophages."xsd:string
http://purl.uniprot.org/citations/27813598http://purl.org/dc/terms/identifier"doi:10.4238/gmr15048889"xsd:string
http://purl.uniprot.org/citations/27813598http://purl.uniprot.org/core/author"Chen J."xsd:string
http://purl.uniprot.org/citations/27813598http://purl.uniprot.org/core/author"Chen Q."xsd:string
http://purl.uniprot.org/citations/27813598http://purl.uniprot.org/core/author"Li L."xsd:string
http://purl.uniprot.org/citations/27813598http://purl.uniprot.org/core/author"He Y.Q."xsd:string
http://purl.uniprot.org/citations/27813598http://purl.uniprot.org/core/author"Lu X.J."xsd:string
http://purl.uniprot.org/citations/27813598http://purl.uniprot.org/core/author"Yu C.H."xsd:string
http://purl.uniprot.org/citations/27813598http://purl.uniprot.org/core/author"Shen H.X."xsd:string
http://purl.uniprot.org/citations/27813598http://purl.uniprot.org/core/author"Chu C.Q."xsd:string
http://purl.uniprot.org/citations/27813598http://purl.uniprot.org/core/date"2016"xsd:gYear
http://purl.uniprot.org/citations/27813598http://purl.uniprot.org/core/name"Genet Mol Res"xsd:string
http://purl.uniprot.org/citations/27813598http://purl.uniprot.org/core/title"LECT2 association with macrophage-mediated killing of Helicobacter pylori by activating NF-kappaB and nitric oxide production."xsd:string
http://purl.uniprot.org/citations/27813598http://purl.uniprot.org/core/volume"15"xsd:string
http://purl.uniprot.org/citations/27813598http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/27813598
http://purl.uniprot.org/citations/27813598http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/27813598
http://purl.uniprot.org/uniprot/#_A0A0R4J0R8-mappedCitation-27813598http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/27813598
http://purl.uniprot.org/uniprot/#_A4L9Q2-mappedCitation-27813598http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/27813598
http://purl.uniprot.org/uniprot/#_A0A286YE52-mappedCitation-27813598http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/27813598
http://purl.uniprot.org/uniprot/#_O88803-mappedCitation-27813598http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/27813598
http://purl.uniprot.org/uniprot/#_Q4U113-mappedCitation-27813598http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/27813598
http://purl.uniprot.org/uniprot/#_Q8BTL4-mappedCitation-27813598http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/27813598
http://purl.uniprot.org/uniprot/#_Q04207-mappedCitation-27813598http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/27813598
http://purl.uniprot.org/uniprot/#_Q548Y4-mappedCitation-27813598http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/27813598