| http://purl.uniprot.org/citations/27871088 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27871088 | http://www.w3.org/2000/01/rdf-schema#comment | "Light-induced oxidation of water by photosystem II (PS II) in plants, algae and cyanobacteria has generated most of the dioxygen in the atmosphere. PS II, a membrane-bound multi-subunit pigment protein complex, couples the one-electron photochemistry at the reaction centre with the four-electron redox chemistry of water oxidation at the Mn4CaO5 cluster in the oxygen-evolving complex (OEC). Under illumination, the OEC cycles through five intermediate S-states (S0 to S4), in which S1 is the dark-stable state and S3 is the last semi-stable state before O-O bond formation and O2 evolution. A detailed understanding of the O-O bond formation mechanism remains a challenge, and will require elucidation of both the structures of the OEC in the different S-states and the binding of the two substrate waters to the catalytic site. Here we report the use of femtosecond pulses from an X-ray free electron laser (XFEL) to obtain damage-free, room temperature structures of dark-adapted (S1), two-flash illuminated (2F; S3-enriched), and ammonia-bound two-flash illuminated (2F-NH3; S3-enriched) PS II. Although the recent 1.95 Å resolution structure of PS II at cryogenic temperature using an XFEL provided a damage-free view of the S1 state, measurements at room temperature are required to study the structural landscape of proteins under functional conditions, and also for in situ advancement of the S-states. To investigate the water-binding site(s), ammonia, a water analogue, has been used as a marker, as it binds to the Mn4CaO5 cluster in the S2 and S3 states. Since the ammonia-bound OEC is active, the ammonia-binding Mn site is not a substrate water site. This approach, together with a comparison of the native dark and 2F states, is used to discriminate between proposed O-O bond formation mechanisms."xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.org/dc/terms/identifier | "doi:10.1038/nature20161"xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.uniprot.org/core/author | "Adams P.D."xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.uniprot.org/core/author | "Afonine P.V."xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.uniprot.org/core/author | "Brunger A.T."xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.uniprot.org/core/author | "Zhu D."xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.uniprot.org/core/author | "Zhang M."xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.uniprot.org/core/author | "Zwart P.H."xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.uniprot.org/core/author | "Young I.D."xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.uniprot.org/core/author | "Robinson J."xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.uniprot.org/core/author | "Nelson S."xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.uniprot.org/core/author | "Boutet S."xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.uniprot.org/core/author | "Koglin J.E."xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.uniprot.org/core/author | "Kubin M."xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.uniprot.org/core/author | "Brewster A.S."xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.uniprot.org/core/author | "Aller P."xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.uniprot.org/core/author | "Fuller F."xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.uniprot.org/core/author | "Weis W.I."xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.uniprot.org/core/author | "Dobbek H."xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.uniprot.org/core/author | "Kern J."xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.uniprot.org/core/author | "Zouni A."xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.uniprot.org/core/author | "Yano J."xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.uniprot.org/core/author | "Bergmann U."xsd:string |
| http://purl.uniprot.org/citations/27871088 | http://purl.uniprot.org/core/author | "Hunter M.S."xsd:string |