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http://purl.uniprot.org/citations/27892520http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/27892520http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/27892520http://www.w3.org/2000/01/rdf-schema#comment"Many experimental techniques rely on specific recognition and stringent binding of proteins by antibodies. This can readily be achieved by introducing an epitope tag. We employed an approach that uses a relative lack of evolutionary conservation to inform epitope tag site selection, followed by integration of the tag-coding sequence into the endogenous locus in zebrafish. We demonstrate that an internal epitope tag is accessible for antibody binding, and that tagged proteins retain wild type function."xsd:string
http://purl.uniprot.org/citations/27892520http://purl.org/dc/terms/identifier"doi:10.1038/srep36986"xsd:string
http://purl.uniprot.org/citations/27892520http://purl.uniprot.org/core/author"Zhang K."xsd:string
http://purl.uniprot.org/citations/27892520http://purl.uniprot.org/core/author"Zhang K."xsd:string
http://purl.uniprot.org/citations/27892520http://purl.uniprot.org/core/author"Balciunas D."xsd:string
http://purl.uniprot.org/citations/27892520http://purl.uniprot.org/core/author"Balciunas D."xsd:string
http://purl.uniprot.org/citations/27892520http://purl.uniprot.org/core/author"Bellipanni G."xsd:string
http://purl.uniprot.org/citations/27892520http://purl.uniprot.org/core/author"Bellipanni G."xsd:string
http://purl.uniprot.org/citations/27892520http://purl.uniprot.org/core/author"Grajevskaja V."xsd:string
http://purl.uniprot.org/citations/27892520http://purl.uniprot.org/core/author"Grajevskaja V."xsd:string
http://purl.uniprot.org/citations/27892520http://purl.uniprot.org/core/author"Burg L."xsd:string
http://purl.uniprot.org/citations/27892520http://purl.uniprot.org/core/author"Burg L."xsd:string
http://purl.uniprot.org/citations/27892520http://purl.uniprot.org/core/author"Bonawitz T."xsd:string
http://purl.uniprot.org/citations/27892520http://purl.uniprot.org/core/author"Bonawitz T."xsd:string
http://purl.uniprot.org/citations/27892520http://purl.uniprot.org/core/author"Waring R."xsd:string
http://purl.uniprot.org/citations/27892520http://purl.uniprot.org/core/author"Waring R."xsd:string
http://purl.uniprot.org/citations/27892520http://purl.uniprot.org/core/date"2016"xsd:gYear
http://purl.uniprot.org/citations/27892520http://purl.uniprot.org/core/date"2016"xsd:gYear
http://purl.uniprot.org/citations/27892520http://purl.uniprot.org/core/name"Sci. Rep."xsd:string
http://purl.uniprot.org/citations/27892520http://purl.uniprot.org/core/name"Sci Rep"xsd:string
http://purl.uniprot.org/citations/27892520http://purl.uniprot.org/core/pages"36986"xsd:string
http://purl.uniprot.org/citations/27892520http://purl.uniprot.org/core/pages"36986"xsd:string
http://purl.uniprot.org/citations/27892520http://purl.uniprot.org/core/title"Internal epitope tagging informed by relative lack of sequence conservation."xsd:string