http://purl.uniprot.org/citations/27905925 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/27905925 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundAbnormal proliferation and migration of vascular smooth muscle cells (VSMCs) is a major contributor to the development of atherosclerotic process. In a previous work, we demonstrated that the insulin receptor isoform A (IRA) and its association with the insulin-like growth factor-I receptor (IGF-IR) confer a proliferative advantage to VSMCs. However, the role of IR and IGF-IR in VSMC migration remains poorly understood.MethodsWound healing assays were performed in VSMCs bearing IR (IRLoxP+/+ VSMCs), or not (IR-/- VSMCs), expressing IRA (IRA VSMCs) or expressing IRB (IRB VSMCs). To study the role of IR isoforms and IGF-IR in experimental atherosclerosis, we used ApoE-/- mice at 8, 12, 18 and 24 weeks of age. Finally, we analyzed the mRNA expression of total IR, IRB isoform, IGF-IR and IGFs by qRT-PCR in the medial layer of human aortas.ResultsIGF-I strongly induced migration of the four cell lines through IGF-IR. In contrast, insulin and IGF-II only caused a significant increase of IRA VSMC migration which might be favored by the formation of IRA/IGF-IR receptors. Additionally, a specific IGF-IR inhibitor, picropodophyllin, completely abolished insulin- and IGF-II-induced migration in IRB, but not in IRA VSMCs. A significant increase of IRA and IGF-IR, and VSMC migration were observed in fibrous plaques from 24-week-old ApoE-/- mice. Finally, we observed a marked increase of IGF-IR, IGF-I and IGF-II in media from fatty streaks as compared with both healthy aortas and fibrolipidic lesions, favoring the ability of medial VSMCs to migrate into the intima.ConclusionsOur data suggest that overexpression of IGF-IR or IRA isoform, as homodimers or as part of IRA/IGF-IR hybrid receptors, confers a stronger migratory capability to VSMCs as might occur in early stages of atherosclerotic process."xsd:string |
http://purl.uniprot.org/citations/27905925 | http://purl.org/dc/terms/identifier | "doi:10.1186/s12933-016-0477-3"xsd:string |
http://purl.uniprot.org/citations/27905925 | http://purl.uniprot.org/core/author | "Michel J.B."xsd:string |
http://purl.uniprot.org/citations/27905925 | http://purl.uniprot.org/core/author | "Fernandez S."xsd:string |
http://purl.uniprot.org/citations/27905925 | http://purl.uniprot.org/core/author | "Escribano O."xsd:string |
http://purl.uniprot.org/citations/27905925 | http://purl.uniprot.org/core/author | "Benito M."xsd:string |
http://purl.uniprot.org/citations/27905925 | http://purl.uniprot.org/core/author | "Egido J."xsd:string |
http://purl.uniprot.org/citations/27905925 | http://purl.uniprot.org/core/author | "Gomez-Hernandez A."xsd:string |
http://purl.uniprot.org/citations/27905925 | http://purl.uniprot.org/core/author | "Martin-Ventura J.L."xsd:string |
http://purl.uniprot.org/citations/27905925 | http://purl.uniprot.org/core/author | "Fernandez-Garcia C.E."xsd:string |
http://purl.uniprot.org/citations/27905925 | http://purl.uniprot.org/core/author | "Beneit N."xsd:string |
http://purl.uniprot.org/citations/27905925 | http://purl.uniprot.org/core/author | "Garcia-Gomez G."xsd:string |
http://purl.uniprot.org/citations/27905925 | http://purl.uniprot.org/core/author | "Perdomo L."xsd:string |
http://purl.uniprot.org/citations/27905925 | http://purl.uniprot.org/core/author | "Diaz-Castroverde S."xsd:string |
http://purl.uniprot.org/citations/27905925 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
http://purl.uniprot.org/citations/27905925 | http://purl.uniprot.org/core/name | "Cardiovasc Diabetol"xsd:string |
http://purl.uniprot.org/citations/27905925 | http://purl.uniprot.org/core/pages | "161"xsd:string |
http://purl.uniprot.org/citations/27905925 | http://purl.uniprot.org/core/title | "Expression of insulin receptor (IR) A and B isoforms, IGF-IR, and IR/IGF-IR hybrid receptors in vascular smooth muscle cells and their role in cell migration in atherosclerosis."xsd:string |
http://purl.uniprot.org/citations/27905925 | http://purl.uniprot.org/core/volume | "15"xsd:string |
http://purl.uniprot.org/citations/27905925 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27905925 |
http://purl.uniprot.org/citations/27905925 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27905925 |
http://purl.uniprot.org/uniprot/#_E9QNX9-mappedCitation-27905925 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27905925 |
http://purl.uniprot.org/uniprot/#_O35466-mappedCitation-27905925 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27905925 |
http://purl.uniprot.org/uniprot/#_B8Q3N4-mappedCitation-27905925 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27905925 |