| http://purl.uniprot.org/citations/27929418 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27929418 | http://www.w3.org/2000/01/rdf-schema#comment | "RTA, the catalytic A-subunit of the ribosome inactivating A/B toxin ricin, inhibits eukaryotic protein biosynthesis by depurination of 28S rRNA. Although cell surface binding of ricin holotoxin is mainly mediated through its B-subunit (RTB), sole application of RTA is also toxic, albeit to a significantly lower extent, suggesting alternative pathways for toxin uptake and transport. Since ricin toxin trafficking in mammalian cells is still not fully understood, we developed a GFP-based reporter assay in yeast that allows rapid identification of cellular components required for RTA uptake and subsequent transport through a target cell. We hereby show that Ypt6p, Sft2p and GARP-complex components play an important role in RTA transport, while neither the retromer complex nor COPIB vesicles are part of the transport machinery. Analyses of yeast knock-out mutants with chromosomal deletion in genes whose products regulate ADP-ribosylation factor GTPases (Arf-GTPases) and/or retrograde Golgi-to-ER (endoplasmic reticulum) transport identified Sso1p, Snc1p, Rer1p, Sec22p, Erv46p, Gea1p and Glo3p as novel components in RTA transport, suggesting the developed reporter assay as a powerful tool to dissect the multistep processes of host cell intoxication in yeast."xsd:string |
| http://purl.uniprot.org/citations/27929418 | http://purl.org/dc/terms/identifier | "doi:10.3390/toxins8120366"xsd:string |
| http://purl.uniprot.org/citations/27929418 | http://purl.uniprot.org/core/author | "Becker B."xsd:string |
| http://purl.uniprot.org/citations/27929418 | http://purl.uniprot.org/core/author | "Schmitt M.J."xsd:string |
| http://purl.uniprot.org/citations/27929418 | http://purl.uniprot.org/core/author | "Schnoder T."xsd:string |
| http://purl.uniprot.org/citations/27929418 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27929418 | http://purl.uniprot.org/core/name | "Toxins (Basel)"xsd:string |
| http://purl.uniprot.org/citations/27929418 | http://purl.uniprot.org/core/pages | "E366"xsd:string |
| http://purl.uniprot.org/citations/27929418 | http://purl.uniprot.org/core/title | "Yeast Reporter Assay to Identify Cellular Components of Ricin Toxin A Chain Trafficking."xsd:string |
| http://purl.uniprot.org/citations/27929418 | http://purl.uniprot.org/core/volume | "8"xsd:string |
| http://purl.uniprot.org/citations/27929418 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27929418 |
| http://purl.uniprot.org/citations/27929418 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27929418 |
| http://purl.uniprot.org/uniprot/#_P16664-mappedCitation-27929418 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27929418 |
| http://purl.uniprot.org/uniprot/#_P38166-mappedCitation-27929418 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27929418 |
| http://purl.uniprot.org/uniprot/#_P38307-mappedCitation-27929418 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27929418 |
| http://purl.uniprot.org/uniprot/#_P34110-mappedCitation-27929418 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27929418 |
| http://purl.uniprot.org/uniprot/#_P39727-mappedCitation-27929418 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27929418 |
| http://purl.uniprot.org/uniprot/#_P39904-mappedCitation-27929418 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27929418 |
| http://purl.uniprot.org/uniprot/#_Q08109-mappedCitation-27929418 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27929418 |
| http://purl.uniprot.org/uniprot/#_Q08144-mappedCitation-27929418 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27929418 |
| http://purl.uniprot.org/uniprot/#_Q12071-mappedCitation-27929418 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27929418 |
| http://purl.uniprot.org/uniprot/#_P22214-mappedCitation-27929418 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27929418 |
| http://purl.uniprot.org/uniprot/#_P40335-mappedCitation-27929418 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27929418 |
| http://purl.uniprot.org/uniprot/#_P40509-mappedCitation-27929418 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27929418 |