| http://purl.uniprot.org/citations/27933483 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27933483 | http://www.w3.org/2000/01/rdf-schema#comment | "Human 17β-hydroxysteroid dehydrogenase type 1 (17β-HSD1), a potential target in breast cancer prevention and therapy, was extracted from human placenta and immobilized on nonporous silica (∼5 μm) with a covalent method for the first time. The optimum initial enzyme concentration and immobilization time during the immobilization process were 0.42 mg mL-1 and 12 h, repectively. The binding was confirmed by scanning electron microscope (SEM) and infrared spectroscopy (FT-IR). It could improve the pH, thermal and storage stability compared to free enzyme. Moreover, the immobilized enzyme could be reused at least four times. A screening method based on it coupled with liquid chromatography-time-of-flight mass spectrometer (LC-TOF/MS) was established, and the half-maximal inhibitory concentration (IC 50) of apigenin for the immobilized enzyme was 291 nM. Subsequently, 10 natural products were evaluated leading to inhibition of the activity of 17β-HSD1 at the concentration of 25 μM, and six of them inhibit the activity over 50%."xsd:string |
| http://purl.uniprot.org/citations/27933483 | http://purl.org/dc/terms/identifier | "doi:10.1007/s12010-016-2339-6"xsd:string |
| http://purl.uniprot.org/citations/27933483 | http://purl.uniprot.org/core/author | "Bai Y."xsd:string |
| http://purl.uniprot.org/citations/27933483 | http://purl.uniprot.org/core/author | "Zhang Q."xsd:string |
| http://purl.uniprot.org/citations/27933483 | http://purl.uniprot.org/core/author | "Yu C."xsd:string |
| http://purl.uniprot.org/citations/27933483 | http://purl.uniprot.org/core/author | "Di B."xsd:string |
| http://purl.uniprot.org/citations/27933483 | http://purl.uniprot.org/core/author | "Mu X.M."xsd:string |
| http://purl.uniprot.org/citations/27933483 | http://purl.uniprot.org/core/author | "Zhou W.D."xsd:string |
| http://purl.uniprot.org/citations/27933483 | http://purl.uniprot.org/core/author | "Su M.X."xsd:string |
| http://purl.uniprot.org/citations/27933483 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/27933483 | http://purl.uniprot.org/core/name | "Appl Biochem Biotechnol"xsd:string |
| http://purl.uniprot.org/citations/27933483 | http://purl.uniprot.org/core/pages | "482-494"xsd:string |
| http://purl.uniprot.org/citations/27933483 | http://purl.uniprot.org/core/title | "Covalent Immobilization of Human Placental 17beta-Hydroxysteroid Dehydrogenase Type 1 onto Glutaraldehyde Activated Silica Coupled with LC-TOF/MS for Anti-Cancer Drug Screening Applications."xsd:string |
| http://purl.uniprot.org/citations/27933483 | http://purl.uniprot.org/core/volume | "182"xsd:string |
| http://purl.uniprot.org/citations/27933483 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27933483 |
| http://purl.uniprot.org/citations/27933483 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27933483 |
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