| http://purl.uniprot.org/citations/27943541 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27943541 | http://www.w3.org/2000/01/rdf-schema#comment | "Essentials The main receptor for platelet glycoprotein (GP) Ibα is von Willebrand factor (VWF). P-selectin and thrombospondin-1 (TSP1) have been suggested as counter receptors for GPIbα. In a laser injury model, P-selectin promotes thrombus propagation independently of VWF and TSP1. In a laser injury model, thrombus persists in interleukin-4 receptor α/GPIbα-transgenic mice.SummaryBackground P-selectin and thrombospondin-1 (TSP1) have been suggested as counter ligands that may mediate GPIbα-dependent thrombus growth independently of von Willebrand factor (VWF) in vitro. However, residual thrombus formation still persists in Vwf -/- Tsp1-/- mice, suggesting existence of other mechanisms that modulate thrombus propagation. Objective We determined whether P-selectin modulates thrombus propagation in injured arterioles independently of TSP1 and VWF. Methods CD-62P blocking antibody in Vwf -/- Tsp1-/- mice was used to inhibit P-selectin. We determined thrombus growth kinetics in two models of thrombosis: FeCl3 injury-induced and laser injury-induced thrombosis. Results In a 10% FeCl3 injury-induced thrombosis model, the initial platelet adhesion, time to form first thrombus, and non-occlusive residual thrombus growth kinetics were comparable between P-selectin-blocking antibody-treated Vwf -/- Tsp1-/- mice and control IgG-treated Vwf -/- Tsp1-/- mice. On the other hand, in a laser injury-induced thrombosis model, residual thrombus growth kinetics were significantly decreased in P-selectin-blocking antibody-treated Vwf -/- Tsp1-/- mice vs. control IgG-treated Vwf -/- Tsp1-/- mice. Because P-selectin has been suggested as a counter ligand for platelet GPIbα, we determined the role of GPIbα in a laser injury-induced thrombosis model. Surprisingly, in a laser injury model, unlike in a FeCl3 injury model, thrombus formation was not completely inhibited in IL4Rα/GPIbα-tg mice. Residual thrombus growth kinetics were comparable between P-selectin-blocking antibody-treated IL4Rα/GPIbα-tg mice and control IgG-treated IL4Rα/GPIbα-tg mice. Comparison of slopes over time showed that residual thrombus growth kinetics were comparable in P-selectin-blocking antibody-treated Vwf -/- Tsp1-/- and control IgG-treated IL4Rα/GPIbα-tg mice Conclusion In a laser injury-induced thrombosis model, P-selectin modulates thrombus propagation independently of VWF and TSP1."xsd:string |
| http://purl.uniprot.org/citations/27943541 | http://purl.org/dc/terms/identifier | "doi:10.1111/jth.13586"xsd:string |
| http://purl.uniprot.org/citations/27943541 | http://purl.uniprot.org/core/author | "Chauhan A.K."xsd:string |
| http://purl.uniprot.org/citations/27943541 | http://purl.uniprot.org/core/author | "Prakash P."xsd:string |
| http://purl.uniprot.org/citations/27943541 | http://purl.uniprot.org/core/author | "Nayak M.K."xsd:string |
| http://purl.uniprot.org/citations/27943541 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/27943541 | http://purl.uniprot.org/core/name | "J Thromb Haemost"xsd:string |
| http://purl.uniprot.org/citations/27943541 | http://purl.uniprot.org/core/pages | "388-394"xsd:string |
| http://purl.uniprot.org/citations/27943541 | http://purl.uniprot.org/core/title | "P-selectin can promote thrombus propagation independently of both von Willebrand factor and thrombospondin-1 in mice."xsd:string |
| http://purl.uniprot.org/citations/27943541 | http://purl.uniprot.org/core/volume | "15"xsd:string |
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