| http://purl.uniprot.org/citations/27975100 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27975100 | http://www.w3.org/2000/01/rdf-schema#comment | "Megakaryocyte (MK)-derived miRNAs have been detected in platelets. Here, we analysed the expression of platelet and circulating miR-223, miR-26b, miR-126 and miR-140 that might be altered with their target mRNAs in type 2 diabetes mellitus (DM2). MiRNAs were isolated from leukocyte-depleted platelets and plasma samples obtained from 28 obese DM2, 19 non-DM obese and 23 healthy individuals. The effect of hyperglycaemia on miRNAs was also evaluated in MKs using MEG-01 and K562 cells under hyperglycaemic conditions after 8 hours up to four weeks. Quantitation of mature miRNA, pre-miRNAs and target mRNA levels (P2RY12 and SELP) were measured by RT-qPCR. To prove the association of miR-26b and miR-140 with SELP (P-selectin) mRNA level, overexpression or inhibition of these miRNAs in MEG-01 MKs was performed using mimics or anti-miRNAs, respectively. The contribution of calpain substrate Dicer to modulation of miRNAs was studied by calpain inhibition. Platelet activation was evaluated via surface P-selectin by flow cytometry. Mature and pre-forms of investigated miRNAs were significantly reduced in DM2, and platelet P2RY12 and SELP mRNA levels were elevated by two-fold at increased platelet activation compared to controls. Significantly blunted miRNA expressions were observed by hyperglycaemia in MEG-01 and K562-MK cells versus baseline values, while the manipulation of miR-26b and miR-140 expression affected SELP mRNA level. Calpeptin pretreatment restored miRNA levels in hyperglycaemic MKs. Overall, miR-223, miR-26b, miR-126 and miR-140 are expressed at a lower level in platelets and MKs in DM2 causing upregulation of P2RY12 and SELP mRNAs that may contribute to adverse platelet function."xsd:string |
| http://purl.uniprot.org/citations/27975100 | http://purl.org/dc/terms/identifier | "doi:10.1160/th16-04-0322"xsd:string |
| http://purl.uniprot.org/citations/27975100 | http://purl.uniprot.org/core/author | "Kunapuli S.P."xsd:string |
| http://purl.uniprot.org/citations/27975100 | http://purl.uniprot.org/core/author | "Czimmerer Z."xsd:string |
| http://purl.uniprot.org/citations/27975100 | http://purl.uniprot.org/core/author | "Poliska S."xsd:string |
| http://purl.uniprot.org/citations/27975100 | http://purl.uniprot.org/core/author | "Kappelmayer J."xsd:string |
| http://purl.uniprot.org/citations/27975100 | http://purl.uniprot.org/core/author | "Kaplar M."xsd:string |
| http://purl.uniprot.org/citations/27975100 | http://purl.uniprot.org/core/author | "Nagy B. Jr."xsd:string |
| http://purl.uniprot.org/citations/27975100 | http://purl.uniprot.org/core/author | "Penyige A."xsd:string |
| http://purl.uniprot.org/citations/27975100 | http://purl.uniprot.org/core/author | "Fejes Z."xsd:string |
| http://purl.uniprot.org/citations/27975100 | http://purl.uniprot.org/core/author | "Beke Debreceni I."xsd:string |
| http://purl.uniprot.org/citations/27975100 | http://purl.uniprot.org/core/author | "Gal Szabo G."xsd:string |
| http://purl.uniprot.org/citations/27975100 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/27975100 | http://purl.uniprot.org/core/name | "Thromb Haemost"xsd:string |
| http://purl.uniprot.org/citations/27975100 | http://purl.uniprot.org/core/pages | "529-542"xsd:string |
| http://purl.uniprot.org/citations/27975100 | http://purl.uniprot.org/core/title | "Hyperglycaemia suppresses microRNA expression in platelets to increase P2RY12 and SELP levels in type 2 diabetes mellitus."xsd:string |
| http://purl.uniprot.org/citations/27975100 | http://purl.uniprot.org/core/volume | "117"xsd:string |
| http://purl.uniprot.org/citations/27975100 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27975100 |
| http://purl.uniprot.org/citations/27975100 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27975100 |
| http://purl.uniprot.org/uniprot/#_B2RE67-mappedCitation-27975100 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27975100 |
| http://purl.uniprot.org/uniprot/#_A0A2H4P6E7-mappedCitation-27975100 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27975100 |
| http://purl.uniprot.org/uniprot/#_B4E183-mappedCitation-27975100 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27975100 |
| http://purl.uniprot.org/uniprot/#_A8K7T1-mappedCitation-27975100 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27975100 |
| http://purl.uniprot.org/uniprot/#_Q9H244-mappedCitation-27975100 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27975100 |