| http://purl.uniprot.org/citations/27977785 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/27977785 | http://www.w3.org/2000/01/rdf-schema#comment | "The pathology of proliferative vitreoretinopathy and proliferative diabetic retinopathy is linked to proliferation, migration, and adhesion of the retinal pigment epithelium. MicroRNA-34a (miR-34a) expression modulates changes in proliferation and migration of retinal pigment epithelial cell line ARPE-19. In this study, we determined that miR-34a interacts with LGR4, identified by bioinformatics using TargetScan Human 5.0, to affect these changes. Double luciferase gene reporter assay confirmed miR-34a involvement in mediating control. miR-34a mimic transfection decreased LGR4 expression. Western blot analysis documented corresponding protein expression inhibition. MTS, Ki67 immunostaining, scratch and transwell testing, along with attachment assay showed that miR-34a upregulation inhibited ARPE-19 cell proliferation, migration and attachment partly through downregulation of LGR4 protein expression. Western blot analysis revealed that both miR-34a upregulation and LGR4 downregulation induced declines in E2F1, p-CDC2, CDK2, CDK4 and CDK6 protein expression. Taken together, miR-34a gene expression upregulation inhibits ARPE-19 cell proliferation, migration and adhesion partly by suppressing LGR4 expression. These results substantiate earlier indications that both miR-34a and LGR4 are potential drug targets to prevent fibrosis in a clinical setting."xsd:string |
| http://purl.uniprot.org/citations/27977785 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0168320"xsd:string |
| http://purl.uniprot.org/citations/27977785 | http://purl.uniprot.org/core/author | "Chen F."xsd:string |
| http://purl.uniprot.org/citations/27977785 | http://purl.uniprot.org/core/author | "Chen X."xsd:string |
| http://purl.uniprot.org/citations/27977785 | http://purl.uniprot.org/core/author | "Zhou L."xsd:string |
| http://purl.uniprot.org/citations/27977785 | http://purl.uniprot.org/core/author | "Xu A."xsd:string |
| http://purl.uniprot.org/citations/27977785 | http://purl.uniprot.org/core/author | "Tang J."xsd:string |
| http://purl.uniprot.org/citations/27977785 | http://purl.uniprot.org/core/author | "Zheng D."xsd:string |
| http://purl.uniprot.org/citations/27977785 | http://purl.uniprot.org/core/author | "Hou Q."xsd:string |
| http://purl.uniprot.org/citations/27977785 | http://purl.uniprot.org/core/author | "Dong X.D."xsd:string |
| http://purl.uniprot.org/citations/27977785 | http://purl.uniprot.org/core/author | "Ma N."xsd:string |
| http://purl.uniprot.org/citations/27977785 | http://purl.uniprot.org/core/author | "Tu L."xsd:string |
| http://purl.uniprot.org/citations/27977785 | http://purl.uniprot.org/core/author | "Tang J.'"xsd:string |
| http://purl.uniprot.org/citations/27977785 | http://purl.uniprot.org/core/date | "2016"xsd:gYear |
| http://purl.uniprot.org/citations/27977785 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/27977785 | http://purl.uniprot.org/core/pages | "e0168320"xsd:string |
| http://purl.uniprot.org/citations/27977785 | http://purl.uniprot.org/core/title | "LGR4 Is a Direct Target of MicroRNA-34a and Modulates the Proliferation and Migration of Retinal Pigment Epithelial ARPE-19 Cells."xsd:string |
| http://purl.uniprot.org/citations/27977785 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/27977785 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/27977785 |
| http://purl.uniprot.org/citations/27977785 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/27977785 |
| http://purl.uniprot.org/uniprot/#_Q59ER8-mappedCitation-27977785 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27977785 |
| http://purl.uniprot.org/uniprot/#_Q9BXB1-mappedCitation-27977785 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/27977785 |
| http://purl.uniprot.org/uniprot/Q9BXB1 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/27977785 |
| http://purl.uniprot.org/uniprot/Q59ER8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/27977785 |