| http://purl.uniprot.org/citations/28051330 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28051330 | http://www.w3.org/2000/01/rdf-schema#comment | "Heterotrimeric G proteins, which are composed of Gα and Gβγ subunits, transduce signals sensed by the coupled surface receptors. Aberrant expressions of G proteins have been observed in many cancer types. This study aimed to determine the expression level of the stimulatory G protein alpha S subunit (Gαs, the main transcript encoded by the GNAS locus) and its biological function in renal cell carcinoma (RCC). Western blotting and quantitative reverse transcription-PCR results show that Gαs expression dramatically increased in RCC cell lines (ACHN, GRC-1, and 786-O) compared to normal renal epithelial cells HK-2. Knockdown of Gαs by small interfering RNA (siRNA) caused a significant inhibition on proliferation of ACHN cells as indicated by MTT assay and colony formation assay. Overexpression of Gαs in HK-2 cells promoted cell proliferation and led to a higher level of intracellular cyclic adenosine monophosphate (cAMP) in response to parathyroid hormone (PTH) compared to the cells transfected with empty vector. Notably, the growth of HK-2 cells overexpressing Gαs was efficiently inhibited in the presence of protein kinase A (PKA) inhibitor H89. Furthermore, in a xenograft model by subcutaneous injection of ACHN cells, tumor growth was also suppressed by H89. Taken together, these results suggest that Gαs plays a tumor-promoting role in RCC and possibly acts through a PKA-dependent pathway. Our findings may provide new clues for target therapy for RCC in the future."xsd:string |
| http://purl.uniprot.org/citations/28051330 | http://purl.org/dc/terms/identifier | "doi:10.1089/dna.2016.3535"xsd:string |
| http://purl.uniprot.org/citations/28051330 | http://purl.uniprot.org/core/author | "Fu Z."xsd:string |
| http://purl.uniprot.org/citations/28051330 | http://purl.uniprot.org/core/author | "Jiang Y."xsd:string |
| http://purl.uniprot.org/citations/28051330 | http://purl.uniprot.org/core/author | "Zhai L."xsd:string |
| http://purl.uniprot.org/citations/28051330 | http://purl.uniprot.org/core/author | "Zhang B."xsd:string |
| http://purl.uniprot.org/citations/28051330 | http://purl.uniprot.org/core/author | "Yao Z."xsd:string |
| http://purl.uniprot.org/citations/28051330 | http://purl.uniprot.org/core/author | "Mu X."xsd:string |
| http://purl.uniprot.org/citations/28051330 | http://purl.uniprot.org/core/author | "Sun N."xsd:string |
| http://purl.uniprot.org/citations/28051330 | http://purl.uniprot.org/core/author | "Zhi L."xsd:string |
| http://purl.uniprot.org/citations/28051330 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28051330 | http://purl.uniprot.org/core/name | "DNA Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/28051330 | http://purl.uniprot.org/core/pages | "237-242"xsd:string |
| http://purl.uniprot.org/citations/28051330 | http://purl.uniprot.org/core/title | "G Protein Alpha S Subunit Promotes Cell Proliferation of Renal Cell Carcinoma with Involvement of Protein Kinase A Signaling."xsd:string |
| http://purl.uniprot.org/citations/28051330 | http://purl.uniprot.org/core/volume | "36"xsd:string |
| http://purl.uniprot.org/citations/28051330 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28051330 |
| http://purl.uniprot.org/citations/28051330 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/28051330 |
| http://purl.uniprot.org/uniprot/#_P63092-mappedCitation-28051330 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28051330 |
| http://purl.uniprot.org/uniprot/#_A0A0S2Z3S5-mappedCitation-28051330 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28051330 |
| http://purl.uniprot.org/uniprot/#_A0A590UJF0-mappedCitation-28051330 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28051330 |
| http://purl.uniprot.org/uniprot/#_A0A0S2Z3H8-mappedCitation-28051330 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28051330 |
| http://purl.uniprot.org/uniprot/#_A0A8V8TL59-mappedCitation-28051330 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28051330 |
| http://purl.uniprot.org/uniprot/#_A0A7I2V5R6-mappedCitation-28051330 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28051330 |
| http://purl.uniprot.org/uniprot/#_A8K8B9-mappedCitation-28051330 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28051330 |