| http://purl.uniprot.org/citations/28059139 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28059139 | http://www.w3.org/2000/01/rdf-schema#comment | "Trichlorophenol (TCP) is a widely used and persistent environmentally toxic compound that poses a carcinogenic risk to humans. Phytoremediation is a proficient cleanup technology for organic pollutants. In this study, we found that the disulfide isomerase-like protein AtPDIL1-2 in plants is a good candidate for enhancing 2,4,6-TCP phytoremediation. The expression of AtPDIL1-2 in Arabidopsis was induced by 2,4,6-TCP. The heterologously expressed AtPDIL1-2 in Escherichia coli exhibited both oxidase and isomerase activities as protein disulfide isomerase and improved bacteria tolerance to 2,4,6-TCP. Further research revealed that transgenic tobacco overexpressing AtPDIL1-2 was more tolerant to high concentrations of 2,4,6-TCP and removed the toxic compound at far greater rates than the control plants. To elucidate the mechanism of action of AtPDIL1-2, we investigated the chemical interaction of AtPDIL1-2 with 2,4,6-TCP for the first time. HPLC analysis implied that AtPDIL1-2 exerts a TCP-binding activity. A suitable configuration of AtPDIL1-2-TCP binding was obtained by molecular docking studies using the AutoDock program. It predicted that the TCP binding site is located in the b-b' domain of AtPDIL1-2 and that His254 of the protein is critical for the binding interaction. These findings imply that AtPDIL1-2 can be used for TCP detoxification by the way of overexpression in plants."xsd:string |
| http://purl.uniprot.org/citations/28059139 | http://purl.org/dc/terms/identifier | "doi:10.1038/srep40130"xsd:string |
| http://purl.uniprot.org/citations/28059139 | http://purl.uniprot.org/core/author | "Qiu J."xsd:string |
| http://purl.uniprot.org/citations/28059139 | http://purl.uniprot.org/core/author | "Xu J."xsd:string |
| http://purl.uniprot.org/citations/28059139 | http://purl.uniprot.org/core/author | "Wang B."xsd:string |
| http://purl.uniprot.org/citations/28059139 | http://purl.uniprot.org/core/author | "Zhu B."xsd:string |
| http://purl.uniprot.org/citations/28059139 | http://purl.uniprot.org/core/author | "Wang L.J."xsd:string |
| http://purl.uniprot.org/citations/28059139 | http://purl.uniprot.org/core/author | "Han H.J."xsd:string |
| http://purl.uniprot.org/citations/28059139 | http://purl.uniprot.org/core/author | "Li Z.J."xsd:string |
| http://purl.uniprot.org/citations/28059139 | http://purl.uniprot.org/core/author | "Gao J.J."xsd:string |
| http://purl.uniprot.org/citations/28059139 | http://purl.uniprot.org/core/author | "Fu X.Y."xsd:string |
| http://purl.uniprot.org/citations/28059139 | http://purl.uniprot.org/core/author | "Peng R.H."xsd:string |
| http://purl.uniprot.org/citations/28059139 | http://purl.uniprot.org/core/author | "Tian Y.S."xsd:string |
| http://purl.uniprot.org/citations/28059139 | http://purl.uniprot.org/core/author | "Yao Q.H."xsd:string |
| http://purl.uniprot.org/citations/28059139 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28059139 | http://purl.uniprot.org/core/name | "Sci Rep"xsd:string |
| http://purl.uniprot.org/citations/28059139 | http://purl.uniprot.org/core/pages | "40130"xsd:string |
| http://purl.uniprot.org/citations/28059139 | http://purl.uniprot.org/core/title | "Disulfide isomerase-like protein AtPDIL1-2 is a good candidate for trichlorophenol phytodetoxification."xsd:string |
| http://purl.uniprot.org/citations/28059139 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/28059139 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28059139 |
| http://purl.uniprot.org/citations/28059139 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/28059139 |
| http://purl.uniprot.org/uniprot/#_Q9SRG3-mappedCitation-28059139 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28059139 |
| http://purl.uniprot.org/uniprot/Q9SRG3 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/28059139 |