| http://purl.uniprot.org/citations/28067406 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28067406 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28067406 | http://www.w3.org/2000/01/rdf-schema#comment | "Ninjurin1 (Ninj1) is a cell surface protein known as a homophilic adhesion molecule. Previous studies have shown a trans-interaction of Ninj1 between immune cells and endothelial cells; however, little is known about Ninj1 modification and structure in the cis-interaction. We showed that Ninj1 assembles into a homomeric complex via a cis-interaction mediated by the intracellular region and N-glycosylation at Asn60 . We identified cis-interaction between Ninj1 proteins using CFP- and YFP-tagged Ninj1 by Förster resonance energy transfer using a confocal microscope and fluorescence-activated cell sorter. We further observed the Ninj1 homomeric complexes composed of two to six monomeric Ninj1 molecules by a formaldehyde cross-linking assay. Co-immunoprecipitation assays with epitope-tagged truncated Ninj1 suggested that the intracellular region encompassing Leu101 -Ala110 participates in Ninj1 homomer assembly. Ninj1 N-glycosylation was characterized by treatment of tunicamycin and substitution of Asn to Gln or Ala. Fluorescence-activated cell sorting-based Förster resonance energy transfer assays further demonstrated that N-glycosylation is indispensable for the Ninj1 cis-interaction, and a formaldehyde cross-linking assay confirmed that interruption of N-glycosylation by Asn substitution disrupted Ninj1 homomeric complex formation. In silico analysis revealed that Ninj1 is highly conserved in vertebrates and that the conserved sequence contains an N-glycosylation motif and cis-interacting intracellular region, which participate in Ninj1 homomer assembly. Taken together, these data show that Ninj1 assembles into a homomeric protein complex and that N-glycosylation is a prerequisite for Ninj1 homomer assembly. J. Cell. Biochem. 118: 2219-2230, 2017. © 2017 Wiley Periodicals, Inc."xsd:string |
| http://purl.uniprot.org/citations/28067406 | http://purl.org/dc/terms/identifier | "doi:10.1002/jcb.25872"xsd:string |
| http://purl.uniprot.org/citations/28067406 | http://purl.org/dc/terms/identifier | "doi:10.1002/jcb.25872"xsd:string |
| http://purl.uniprot.org/citations/28067406 | http://purl.uniprot.org/core/author | "Shin M.W."xsd:string |
| http://purl.uniprot.org/citations/28067406 | http://purl.uniprot.org/core/author | "Shin M.W."xsd:string |
| http://purl.uniprot.org/citations/28067406 | http://purl.uniprot.org/core/author | "Kim R.H."xsd:string |
| http://purl.uniprot.org/citations/28067406 | http://purl.uniprot.org/core/author | "Kim R.H."xsd:string |
| http://purl.uniprot.org/citations/28067406 | http://purl.uniprot.org/core/author | "Shin D."xsd:string |
| http://purl.uniprot.org/citations/28067406 | http://purl.uniprot.org/core/author | "Shin D."xsd:string |
| http://purl.uniprot.org/citations/28067406 | http://purl.uniprot.org/core/author | "Bae S.J."xsd:string |
| http://purl.uniprot.org/citations/28067406 | http://purl.uniprot.org/core/author | "Bae S.J."xsd:string |
| http://purl.uniprot.org/citations/28067406 | http://purl.uniprot.org/core/author | "Kim K.W."xsd:string |
| http://purl.uniprot.org/citations/28067406 | http://purl.uniprot.org/core/author | "Kim K.W."xsd:string |
| http://purl.uniprot.org/citations/28067406 | http://purl.uniprot.org/core/author | "Wee H.J."xsd:string |
| http://purl.uniprot.org/citations/28067406 | http://purl.uniprot.org/core/author | "Wee H.J."xsd:string |
| http://purl.uniprot.org/citations/28067406 | http://purl.uniprot.org/core/author | "Son T."xsd:string |
| http://purl.uniprot.org/citations/28067406 | http://purl.uniprot.org/core/author | "Son T."xsd:string |
| http://purl.uniprot.org/citations/28067406 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28067406 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28067406 | http://purl.uniprot.org/core/name | "J. Cell. Biochem."xsd:string |
| http://purl.uniprot.org/citations/28067406 | http://purl.uniprot.org/core/name | "J. Cell. Biochem."xsd:string |
| http://purl.uniprot.org/citations/28067406 | http://purl.uniprot.org/core/pages | "2219-2230"xsd:string |
| http://purl.uniprot.org/citations/28067406 | http://purl.uniprot.org/core/pages | "2219-2230"xsd:string |