| http://purl.uniprot.org/citations/28075010 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28075010 | http://www.w3.org/2000/01/rdf-schema#comment | "In this study, we examine whether an anti-inflammatory thiourea derivative, compound #326, actions on ion channels. The effects of compound #326 on Ca2+ -activated K+ channels were evaluated by patch-clamp recordings obtained in cell-attached, inside-out or whole-cell configuration. In pituitary GH3 cells, compound #326 increased the amplitude of Ca2+ -activated K+ currents (IK(Ca) ) with an EC50 value of 11.6 μM, which was reversed by verruculogen, but not tolbutamide or TRAM-34. Under inside-out configuration, a bath application of compound #326 raised the probability of large-conductance Ca2+ -activated K+ (BKCa ) channels. The activation curve of BKCa channels was shifted to less depolarised potential with no modification of the gating charge of the curve; consequently, the difference of free energy was reduced in the presence of this compound. Compound #326-stimulated activity of BKCa channels is explained by a shortening of mean closed time, despite its inability to alter single-channel conductance. Neither delayed-rectifier nor erg-mediated K+ currents was modified. Compound #326 decreased the peak amplitude of voltage-gated Na+ current with no clear change in the overall current-voltage relationship of this current. In HEK293T cells expressing α-hSlo, compound #326 enhanced BKCa channels effectively. Intriguingly, the inhibitory actions of compound #326 on interleukin 1β in lipopolysaccharide-activated microglia were significantly reversed by verruculogen, whereas BKCa channel inhibitors suppressed the expressions of inducible nitric oxide synthase. The BKCa channels could be an important target for compound #326 if similar in vivo results occur, and the multi-functionality of BKCa channels in modulating microglial immunity merit further investigation."xsd:string |
| http://purl.uniprot.org/citations/28075010 | http://purl.org/dc/terms/identifier | "doi:10.1002/jcp.25788"xsd:string |
| http://purl.uniprot.org/citations/28075010 | http://purl.uniprot.org/core/author | "Lee C.C."xsd:string |
| http://purl.uniprot.org/citations/28075010 | http://purl.uniprot.org/core/author | "Shen S."xsd:string |
| http://purl.uniprot.org/citations/28075010 | http://purl.uniprot.org/core/author | "Chen H.H."xsd:string |
| http://purl.uniprot.org/citations/28075010 | http://purl.uniprot.org/core/author | "Hsu Y.T."xsd:string |
| http://purl.uniprot.org/citations/28075010 | http://purl.uniprot.org/core/author | "Lai M.C."xsd:string |
| http://purl.uniprot.org/citations/28075010 | http://purl.uniprot.org/core/author | "Chan M.H."xsd:string |
| http://purl.uniprot.org/citations/28075010 | http://purl.uniprot.org/core/author | "Wu S.N."xsd:string |
| http://purl.uniprot.org/citations/28075010 | http://purl.uniprot.org/core/author | "Shie F.S."xsd:string |
| http://purl.uniprot.org/citations/28075010 | http://purl.uniprot.org/core/author | "Chern J.H."xsd:string |
| http://purl.uniprot.org/citations/28075010 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28075010 | http://purl.uniprot.org/core/name | "J Cell Physiol"xsd:string |
| http://purl.uniprot.org/citations/28075010 | http://purl.uniprot.org/core/pages | "3409-3421"xsd:string |
| http://purl.uniprot.org/citations/28075010 | http://purl.uniprot.org/core/title | "Stimulatory actions of a novel thiourea derivative on large-conductance, calcium-activated potassium channels."xsd:string |
| http://purl.uniprot.org/citations/28075010 | http://purl.uniprot.org/core/volume | "232"xsd:string |
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