| http://purl.uniprot.org/citations/28092359 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28092359 | http://www.w3.org/2000/01/rdf-schema#comment | "Substrate channeling has emerged as a common mechanism for enzymatic intermediate transfer. A conspicuous gap in knowledge concerns the use of covalent lysine imines in the transfer of carbonyl-group-containing intermediates, despite their wideuse in enzymatic catalysis. Here we show how imine chemistry operates in the transfer of covalent intermediates in pyridoxal 5'-phosphate biosynthesis by the Arabidopsis thaliana enzyme Pdx1. An initial ribose 5-phosphate lysine imine is converted to the chromophoric I320 intermediate, simultaneously bound to two lysine residues and partially vacating the active site, which creates space for glyceraldehyde 3-phosphate to bind. Crystal structures show how substrate binding, catalysis and shuttling are coupled to conformational changes around strand β6 of the Pdx1 (βα)8-barrel. The dual-specificity active site and imine relay mechanism for migration of carbonyl intermediates provide elegant solutions to the challenge of coordinating a complex sequence of reactions that follow a path of over 20 Å between substrate- and product-binding sites."xsd:string |
| http://purl.uniprot.org/citations/28092359 | http://purl.org/dc/terms/identifier | "doi:10.1038/nchembio.2273"xsd:string |
| http://purl.uniprot.org/citations/28092359 | http://purl.uniprot.org/core/author | "Begley T.P."xsd:string |
| http://purl.uniprot.org/citations/28092359 | http://purl.uniprot.org/core/author | "Ealick S.E."xsd:string |
| http://purl.uniprot.org/citations/28092359 | http://purl.uniprot.org/core/author | "Royant A."xsd:string |
| http://purl.uniprot.org/citations/28092359 | http://purl.uniprot.org/core/author | "Zhang Y."xsd:string |
| http://purl.uniprot.org/citations/28092359 | http://purl.uniprot.org/core/author | "Sinning I."xsd:string |
| http://purl.uniprot.org/citations/28092359 | http://purl.uniprot.org/core/author | "Guedez G."xsd:string |
| http://purl.uniprot.org/citations/28092359 | http://purl.uniprot.org/core/author | "Tews I."xsd:string |
| http://purl.uniprot.org/citations/28092359 | http://purl.uniprot.org/core/author | "Windeisen V."xsd:string |
| http://purl.uniprot.org/citations/28092359 | http://purl.uniprot.org/core/author | "Weber S."xsd:string |
| http://purl.uniprot.org/citations/28092359 | http://purl.uniprot.org/core/author | "Evans G."xsd:string |
| http://purl.uniprot.org/citations/28092359 | http://purl.uniprot.org/core/author | "Strohmeier M."xsd:string |
| http://purl.uniprot.org/citations/28092359 | http://purl.uniprot.org/core/author | "Hanes J.W."xsd:string |
| http://purl.uniprot.org/citations/28092359 | http://purl.uniprot.org/core/author | "Rodrigues M.J."xsd:string |
| http://purl.uniprot.org/citations/28092359 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28092359 | http://purl.uniprot.org/core/name | "Nat Chem Biol"xsd:string |
| http://purl.uniprot.org/citations/28092359 | http://purl.uniprot.org/core/pages | "290-294"xsd:string |
| http://purl.uniprot.org/citations/28092359 | http://purl.uniprot.org/core/title | "Lysine relay mechanism coordinates intermediate transfer in vitamin B6 biosynthesis."xsd:string |
| http://purl.uniprot.org/citations/28092359 | http://purl.uniprot.org/core/volume | "13"xsd:string |
| http://purl.uniprot.org/citations/28092359 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28092359 |
| http://purl.uniprot.org/citations/28092359 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/28092359 |
| http://purl.uniprot.org/uniprot/#_F6M3L4-mappedCitation-28092359 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28092359 |
| http://purl.uniprot.org/uniprot/#_O80448-mappedCitation-28092359 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28092359 |