| http://purl.uniprot.org/citations/28107504 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28107504 | http://www.w3.org/2000/01/rdf-schema#comment | "The neural crest is a source to produce multipotent neural crest stem cells that have a potential to differentiate into diverse cell types. The transcription factor SOX10 is expressed through early neural crest progenitors and stem cells in vertebrates. Here we report the generation of SOX10-Nano-lantern (NL) reporter human induced pluripotent stem cells (hiPS) by using CRISPR/Cas9 systems, that are beneficial to investigate the generation and maintenance of neural crest progenitor cells. SOX10-NL positive cells are produced transiently from hiPS cells by treatment with TGFβ inhibitor SB431542 and GSK3 inhibitor CHIR99021. We found that all SOX10-NL-positive cells expressed an early neural crest marker NGFR, however SOX10-NL-positive cells purified from differentiated hiPS cells progressively attenuate their NL-expression under proliferation. We therefore attempted to maintain SOX10-NL-positive cells with additional signaling on the plane and sphere culture conditions. These SOX10-NL cells provide us to investigate mass culture with neural crest cells for stem cell research."xsd:string |
| http://purl.uniprot.org/citations/28107504 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0170342"xsd:string |
| http://purl.uniprot.org/citations/28107504 | http://purl.uniprot.org/core/author | "Kinoshita S."xsd:string |
| http://purl.uniprot.org/citations/28107504 | http://purl.uniprot.org/core/author | "Shibata M."xsd:string |
| http://purl.uniprot.org/citations/28107504 | http://purl.uniprot.org/core/author | "Sato T."xsd:string |
| http://purl.uniprot.org/citations/28107504 | http://purl.uniprot.org/core/author | "Ueno M."xsd:string |
| http://purl.uniprot.org/citations/28107504 | http://purl.uniprot.org/core/author | "Ohi H."xsd:string |
| http://purl.uniprot.org/citations/28107504 | http://purl.uniprot.org/core/author | "Ikeya M."xsd:string |
| http://purl.uniprot.org/citations/28107504 | http://purl.uniprot.org/core/author | "Sotozono C."xsd:string |
| http://purl.uniprot.org/citations/28107504 | http://purl.uniprot.org/core/author | "Horikiri T."xsd:string |
| http://purl.uniprot.org/citations/28107504 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28107504 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/28107504 | http://purl.uniprot.org/core/pages | "e0170342"xsd:string |
| http://purl.uniprot.org/citations/28107504 | http://purl.uniprot.org/core/title | "SOX10-Nano-Lantern Reporter Human iPS Cells; A Versatile Tool for Neural Crest Research."xsd:string |
| http://purl.uniprot.org/citations/28107504 | http://purl.uniprot.org/core/volume | "12"xsd:string |
| http://purl.uniprot.org/citations/28107504 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28107504 |
| http://purl.uniprot.org/citations/28107504 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/28107504 |
| http://purl.uniprot.org/uniprot/#_P56693-mappedCitation-28107504 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28107504 |
| http://purl.uniprot.org/uniprot/P56693 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/28107504 |