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http://purl.uniprot.org/citations/28117598http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/28117598http://www.w3.org/2000/01/rdf-schema#comment"

Aims

Mitochondrial dysfunction has emerged as a major contributing factor to endothelial dysfunction and vascular disease, but the key mechanisms underlying mitochondrial dysfunction-induced endothelial dysfunction remain to be elucidated. In this study, we aim at determining whether mitochondrial dysfunction in endothelial cells plays a key role in vascular disease, by examining the phenotype of endothelial-specific CR6-interacting factor 1 (CRIF1) knockout mice. We also used siRNA-mediated downregulation of CRIF1 gene in the endothelial cells to study about the in vitro pathophysiological underlying mechanisms.

Results

Downregulation of CRIF1 in endothelial cells caused disturbances of mitochondrial oxidative phosphorylation complexes and membrane potential, leading to enhanced mitochondrial reactive oxygen species production. Gene silencing of CRIF1 results in decreased SIRT1 expression along with increased endothelial nitric oxide synthase (eNOS) acetylation, leading to reduced nitric oxide production both in vitro and in vivo. Endothelium-dependent vasorelaxation of aortic rings from CRIF1 knockout (KO) mice was considerably less than in wild-type mice, and it was partially recovered by Sirt1 overexpression in CRIF1 KO mice.

Innovation

Our results show for the first time a relationship between mitochondrial dysfunction and impaired vascular function induced in CRIF1 deficiency conditions and also the possible underlying pathway involved.

Conclusion

These findings indicate that CRIF1 plays an important role in maintaining mitochondrial and endothelial function through its effects on the SIRT1-eNOS pathway. Antioxid. Redox Signal. 27, 234-249."xsd:string
http://purl.uniprot.org/citations/28117598http://purl.org/dc/terms/identifier"doi:10.1089/ars.2016.6719"xsd:string
http://purl.uniprot.org/citations/28117598http://purl.uniprot.org/core/author"Kim D.W."xsd:string
http://purl.uniprot.org/citations/28117598http://purl.uniprot.org/core/author"Song H.J."xsd:string
http://purl.uniprot.org/citations/28117598http://purl.uniprot.org/core/author"Shong M."xsd:string
http://purl.uniprot.org/citations/28117598http://purl.uniprot.org/core/author"Kang S.K."xsd:string
http://purl.uniprot.org/citations/28117598http://purl.uniprot.org/core/author"Kim C.S."xsd:string
http://purl.uniprot.org/citations/28117598http://purl.uniprot.org/core/author"Choi S.J."xsd:string
http://purl.uniprot.org/citations/28117598http://purl.uniprot.org/core/author"Irani K."xsd:string
http://purl.uniprot.org/citations/28117598http://purl.uniprot.org/core/author"Jung S.B."xsd:string
http://purl.uniprot.org/citations/28117598http://purl.uniprot.org/core/author"Jeon B.H."xsd:string
http://purl.uniprot.org/citations/28117598http://purl.uniprot.org/core/author"Kweon G.R."xsd:string
http://purl.uniprot.org/citations/28117598http://purl.uniprot.org/core/author"Piao S."xsd:string
http://purl.uniprot.org/citations/28117598http://purl.uniprot.org/core/author"Shin N."xsd:string
http://purl.uniprot.org/citations/28117598http://purl.uniprot.org/core/author"Jeong J.O."xsd:string
http://purl.uniprot.org/citations/28117598http://purl.uniprot.org/core/author"Ryu M.J."xsd:string
http://purl.uniprot.org/citations/28117598http://purl.uniprot.org/core/author"Jin S.A."xsd:string
http://purl.uniprot.org/citations/28117598http://purl.uniprot.org/core/author"Nagar H."xsd:string
http://purl.uniprot.org/citations/28117598http://purl.uniprot.org/core/date"2017"xsd:gYear
http://purl.uniprot.org/citations/28117598http://purl.uniprot.org/core/name"Antioxid Redox Signal"xsd:string
http://purl.uniprot.org/citations/28117598http://purl.uniprot.org/core/pages"234-249"xsd:string
http://purl.uniprot.org/citations/28117598http://purl.uniprot.org/core/title"CR6-Interacting Factor 1 Deficiency Impairs Vascular Function by Inhibiting the Sirt1-Endothelial Nitric Oxide Synthase Pathway."xsd:string
http://purl.uniprot.org/citations/28117598http://purl.uniprot.org/core/volume"27"xsd:string
http://purl.uniprot.org/citations/28117598http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/28117598
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