| http://purl.uniprot.org/citations/28128549 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28128549 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28128549 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/28128549 | http://www.w3.org/2000/01/rdf-schema#comment | "The reduction of epoxyqueuosine (oQ) is the last step in the synthesis of the tRNA modification queuosine (Q). While the epoxyqueuosine reductase (EC 1.17.99.6) enzymatic activity was first described 30 years ago, the encoding gene queG was only identified in Escherichia coli in 2011. Interestingly, queG is absent from a large number of sequenced genomes that harbor Q synthesis or salvage genes, suggesting the existence of an alternative epoxyqueuosine reductase in these organisms. By analyzing phylogenetic distributions, physical gene clustering, and fusions, members of the Domain of Unknown Function 208 (DUF208) family were predicted to encode for an alternative epoxyqueuosine reductase. This prediction was validated with genetic methods. The Q modification is present in Lactobacillus salivarius, an organism missing queG but harboring the duf208 gene. Acinetobacter baylyi ADP1 is one of the few organisms that harbor both QueG and DUF208, and deletion of both corresponding genes was required to observe the absence of Q and the accumulation of oQ in tRNA. Finally, the conversion oQ to Q was restored in an E. coli queG mutant by complementation with plasmids harboring duf208 genes from different bacteria. Members of the DUF208 family are not homologous to QueG enzymes, and thus, duf208 is a non-orthologous replacement of queG. We propose to name DUF208 encoding genes as queH. While QueH contains conserved cysteines that could be involved in the coordination of a Fe/S center in a similar fashion to what has been identified in QueG, no cobalamin was identified associated with recombinant QueH protein."xsd:string |
| http://purl.uniprot.org/citations/28128549 | http://purl.org/dc/terms/identifier | "doi:10.1021/acschembio.6b01100"xsd:string |
| http://purl.uniprot.org/citations/28128549 | http://purl.org/dc/terms/identifier | "doi:10.1021/acschembio.6b01100"xsd:string |
| http://purl.uniprot.org/citations/28128549 | http://purl.uniprot.org/core/author | "Chen W.H."xsd:string |
| http://purl.uniprot.org/citations/28128549 | http://purl.uniprot.org/core/author | "Chen W.H."xsd:string |
| http://purl.uniprot.org/citations/28128549 | http://purl.uniprot.org/core/author | "de Crecy-Lagard V."xsd:string |
| http://purl.uniprot.org/citations/28128549 | http://purl.uniprot.org/core/author | "de Crecy-Lagard V."xsd:string |
| http://purl.uniprot.org/citations/28128549 | http://purl.uniprot.org/core/author | "Bruner S.D."xsd:string |
| http://purl.uniprot.org/citations/28128549 | http://purl.uniprot.org/core/author | "Bruner S.D."xsd:string |
| http://purl.uniprot.org/citations/28128549 | http://purl.uniprot.org/core/author | "Limbach P.A."xsd:string |
| http://purl.uniprot.org/citations/28128549 | http://purl.uniprot.org/core/author | "Limbach P.A."xsd:string |
| http://purl.uniprot.org/citations/28128549 | http://purl.uniprot.org/core/author | "Ross R."xsd:string |
| http://purl.uniprot.org/citations/28128549 | http://purl.uniprot.org/core/author | "Ross R."xsd:string |
| http://purl.uniprot.org/citations/28128549 | http://purl.uniprot.org/core/author | "Zallot R."xsd:string |
| http://purl.uniprot.org/citations/28128549 | http://purl.uniprot.org/core/author | "Zallot R."xsd:string |
| http://purl.uniprot.org/citations/28128549 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28128549 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28128549 | http://purl.uniprot.org/core/name | "ACS Chem. Biol."xsd:string |
| http://purl.uniprot.org/citations/28128549 | http://purl.uniprot.org/core/name | "ACS Chem. Biol."xsd:string |
| http://purl.uniprot.org/citations/28128549 | http://purl.uniprot.org/core/pages | "844-851"xsd:string |
| http://purl.uniprot.org/citations/28128549 | http://purl.uniprot.org/core/pages | "844-851"xsd:string |
| http://purl.uniprot.org/citations/28128549 | http://purl.uniprot.org/core/title | "Identification of a novel epoxyqueuosine reductase family by comparative genomics."xsd:string |