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http://purl.uniprot.org/citations/28137312http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/28137312http://www.w3.org/2000/01/rdf-schema#comment"

Background

The mammalian sperm-associated antigen 16 gene (Spag16) uses alternative promoters to produce two major transcript isoforms (Spag16L and Spag16S) and encode proteins that are involved in the cilia/flagella formation and motility. In silico analysis of both mouse and human SPAG16L promoters reveals the existence of multiple putative SOX5 binding sites. Given that the SOX5 gene encodes a 48-kDa transcription factor (S-SOX5) and the presence of putative SOX5 binding sites at the SPAG16L promoter, regulation of SPAG16L expression by S-SOX5 was studied in the present work.

Results

S-SOX5 activated human SPAG16L promoter activity in the human bronchial epithelia cell line BEAS-2B cells. Mutation of S-SOX5 binding sites abolished the stimulatory effect. Overexpression of S-SOX5 resulted in a significant increase in the abundance of SPAG16L transcripts whereas silencing of S-SOX5 by RNAi largely reduced the SPAG16L expression. Chromatin immunoprecipitation assays showed that S-SOX5 directly interacts with the SPAG16L promoter.

Conclusion

S-SOX5 regulates transcription of human SPAG16L gene via directly binding to the promoter of SPAG16L. It has been reported that expression of sperm-associated antigen 6 (SPAG6), encoding another axonemal protein, is activated by S-SOX5. Therefore, S-SOX5 may regulate formation of motile cilia/flagella through globally mediating expression of genes encoding axonemal proteins."xsd:string
http://purl.uniprot.org/citations/28137312http://purl.org/dc/terms/identifier"doi:10.1186/s12867-017-0082-3"xsd:string
http://purl.uniprot.org/citations/28137312http://purl.uniprot.org/core/author"Liu Y."xsd:string
http://purl.uniprot.org/citations/28137312http://purl.uniprot.org/core/author"Li Y."xsd:string
http://purl.uniprot.org/citations/28137312http://purl.uniprot.org/core/author"Liu J."xsd:string
http://purl.uniprot.org/citations/28137312http://purl.uniprot.org/core/author"Li W."xsd:string
http://purl.uniprot.org/citations/28137312http://purl.uniprot.org/core/author"Song S."xsd:string
http://purl.uniprot.org/citations/28137312http://purl.uniprot.org/core/author"Zhang Q."xsd:string
http://purl.uniprot.org/citations/28137312http://purl.uniprot.org/core/author"Zhang Z."xsd:string
http://purl.uniprot.org/citations/28137312http://purl.uniprot.org/core/author"Zhang L."xsd:string
http://purl.uniprot.org/citations/28137312http://purl.uniprot.org/core/author"Min J."xsd:string
http://purl.uniprot.org/citations/28137312http://purl.uniprot.org/core/author"Shuang C."xsd:string
http://purl.uniprot.org/citations/28137312http://purl.uniprot.org/core/date"2017"xsd:gYear
http://purl.uniprot.org/citations/28137312http://purl.uniprot.org/core/name"BMC Mol Biol"xsd:string
http://purl.uniprot.org/citations/28137312http://purl.uniprot.org/core/pages"2"xsd:string
http://purl.uniprot.org/citations/28137312http://purl.uniprot.org/core/title"Transcriptional regulation of human sperm-associated antigen 16 gene by S-SOX5."xsd:string
http://purl.uniprot.org/citations/28137312http://purl.uniprot.org/core/volume"18"xsd:string
http://purl.uniprot.org/citations/28137312http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/28137312
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