| http://purl.uniprot.org/citations/28143931 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28143931 | http://www.w3.org/2000/01/rdf-schema#comment | "Mammalian intrinsic apoptosis requires activation of the initiator caspase-9, which then cleaves and activates the effector caspases to execute cell killing. The heptameric Apaf-1 apoptosome is indispensable for caspase-9 activation by together forming a holoenzyme. The molecular mechanism of caspase-9 activation remains largely enigmatic. Here, we report the cryoelectron microscopy (cryo-EM) structure of an apoptotic holoenzyme and structure-guided biochemical analyses. The caspase recruitment domains (CARDs) of Apaf-1 and caspase-9 assemble in two different ways: a 4:4 complex docks onto the central hub of the apoptosome, and a 2:1 complex binds the periphery of the central hub. The interface between the CARD complex and the central hub is required for caspase-9 activation within the holoenzyme. Unexpectedly, the CARD of free caspase-9 strongly inhibits its proteolytic activity. These structural and biochemical findings demonstrate that the apoptosome activates caspase-9 at least in part through sequestration of the inhibitory CARD domain."xsd:string |
| http://purl.uniprot.org/citations/28143931 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1620626114"xsd:string |
| http://purl.uniprot.org/citations/28143931 | http://purl.uniprot.org/core/author | "Hu Q."xsd:string |
| http://purl.uniprot.org/citations/28143931 | http://purl.uniprot.org/core/author | "Huang W."xsd:string |
| http://purl.uniprot.org/citations/28143931 | http://purl.uniprot.org/core/author | "Li Y."xsd:string |
| http://purl.uniprot.org/citations/28143931 | http://purl.uniprot.org/core/author | "Shi Y."xsd:string |
| http://purl.uniprot.org/citations/28143931 | http://purl.uniprot.org/core/author | "Zhou M."xsd:string |
| http://purl.uniprot.org/citations/28143931 | http://purl.uniprot.org/core/author | "Bai X.C."xsd:string |
| http://purl.uniprot.org/citations/28143931 | http://purl.uniprot.org/core/author | "Scheres S.H."xsd:string |
| http://purl.uniprot.org/citations/28143931 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28143931 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/28143931 | http://purl.uniprot.org/core/pages | "1542-1547"xsd:string |
| http://purl.uniprot.org/citations/28143931 | http://purl.uniprot.org/core/title | "Mechanistic insights into caspase-9 activation by the structure of the apoptosome holoenzyme."xsd:string |
| http://purl.uniprot.org/citations/28143931 | http://purl.uniprot.org/core/volume | "114"xsd:string |
| http://purl.uniprot.org/citations/28143931 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28143931 |
| http://purl.uniprot.org/citations/28143931 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/28143931 |
| http://purl.uniprot.org/uniprot/#_A7E2A2-mappedCitation-28143931 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28143931 |
| http://purl.uniprot.org/uniprot/#_A5YM44-mappedCitation-28143931 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28143931 |
| http://purl.uniprot.org/uniprot/#_A8K7R5-mappedCitation-28143931 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28143931 |
| http://purl.uniprot.org/uniprot/#_A8K7U6-mappedCitation-28143931 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28143931 |
| http://purl.uniprot.org/uniprot/#_O14727-mappedCitation-28143931 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28143931 |
| http://purl.uniprot.org/uniprot/#_F8VVS7-mappedCitation-28143931 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28143931 |
| http://purl.uniprot.org/uniprot/#_P55211-mappedCitation-28143931 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28143931 |
| http://purl.uniprot.org/uniprot/#_P00004-mappedCitation-28143931 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28143931 |