| http://purl.uniprot.org/citations/2820474 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2820474 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2820474 | http://www.w3.org/2000/01/rdf-schema#comment | "Plasminogen activator inhibitor 1 (PAI-1) is the primary inhibitor of tissue-type plasminogen activator and thus performs an essential role in the regulation of the fibrinolytic process. It is a member of a large family of serine protease inhibitors (serpins). We determined the structure of the PAI-1 gene in order to more completely investigate the relationship of PAI-1 to other serpins and, at the same time, to begin to delineate structure-function relations in PAI-1 itself. A human genomic cosmid DNA library was screened and found to contain two independent clones, each harboring the entire PAI-1 gene. Restriction site mapping, electron microscopic inspection of heteroduplexes, and nucleotide sequence analysis all demonstrate that the PAI-1 gene is approximately 12.2 kilobase pairs in length and consists of nine exons and eight introns. All intron-exon boundaries are in accord with the "GT-AG" rule, including a cryptic acceptor splice site found in intron 7. The intron-exon pattern of the PAI-1 gene is distinct from that of most other serpins except that intron 3 of PAI-1 occupies an identical position as intron E of ovalbumin. Comparison of our data with the proposed subdomain structure of serpins suggests that seven of the eight introns may occupy a nonrandom position in the gene. These introns either delineate boundaries of individual structural subdomains or are located in random coil regions of the protein. Transcription of the PAI-1 gene in cultured vascular endothelial cells results in two distinct mRNA species. Our data suggest that these two transcripts arise by alternative polyadenylation."xsd:string |
| http://purl.uniprot.org/citations/2820474 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi00387a004"xsd:string |
| http://purl.uniprot.org/citations/2820474 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi00387a004"xsd:string |
| http://purl.uniprot.org/citations/2820474 | http://purl.uniprot.org/core/author | "van Heerikhuizen H."xsd:string |
| http://purl.uniprot.org/citations/2820474 | http://purl.uniprot.org/core/author | "van Heerikhuizen H."xsd:string |
| http://purl.uniprot.org/citations/2820474 | http://purl.uniprot.org/core/author | "Pannekoek H."xsd:string |
| http://purl.uniprot.org/citations/2820474 | http://purl.uniprot.org/core/author | "Pannekoek H."xsd:string |
| http://purl.uniprot.org/citations/2820474 | http://purl.uniprot.org/core/author | "Keijer J."xsd:string |
| http://purl.uniprot.org/citations/2820474 | http://purl.uniprot.org/core/author | "Keijer J."xsd:string |
| http://purl.uniprot.org/citations/2820474 | http://purl.uniprot.org/core/author | "Linders M."xsd:string |
| http://purl.uniprot.org/citations/2820474 | http://purl.uniprot.org/core/author | "Linders M."xsd:string |
| http://purl.uniprot.org/citations/2820474 | http://purl.uniprot.org/core/author | "Loskutoff D.J."xsd:string |
| http://purl.uniprot.org/citations/2820474 | http://purl.uniprot.org/core/author | "Loskutoff D.J."xsd:string |
| http://purl.uniprot.org/citations/2820474 | http://purl.uniprot.org/core/author | "Veerman H."xsd:string |
| http://purl.uniprot.org/citations/2820474 | http://purl.uniprot.org/core/author | "Veerman H."xsd:string |
| http://purl.uniprot.org/citations/2820474 | http://purl.uniprot.org/core/date | "1987"xsd:gYear |
| http://purl.uniprot.org/citations/2820474 | http://purl.uniprot.org/core/date | "1987"xsd:gYear |
| http://purl.uniprot.org/citations/2820474 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/2820474 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/2820474 | http://purl.uniprot.org/core/pages | "3763-3768"xsd:string |
| http://purl.uniprot.org/citations/2820474 | http://purl.uniprot.org/core/pages | "3763-3768"xsd:string |
| http://purl.uniprot.org/citations/2820474 | http://purl.uniprot.org/core/title | "Structure of the human plasminogen activator inhibitor 1 gene: nonrandom distribution of introns."xsd:string |
| http://purl.uniprot.org/citations/2820474 | http://purl.uniprot.org/core/title | "Structure of the human plasminogen activator inhibitor 1 gene: nonrandom distribution of introns."xsd:string |