| http://purl.uniprot.org/citations/28211790 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28211790 | http://www.w3.org/2000/01/rdf-schema#comment | "Manipulating gene function cell type-specifically is a common experimental goal in Drosophila research and has been central to studies of neural development, circuit computation, and behavior. However, current cell type-specific gene disruption techniques in flies often reduce gene activity incompletely or rely on cell division. Here we describe FlpStop, a generalizable tool for conditional gene disruption and rescue in post-mitotic cells. In proof-of-principle experiments, we manipulated apterous, a regulator of wing development. Next, we produced conditional null alleles of Glutamic acid decarboxylase 1 (Gad1) and Resistant to dieldrin (Rdl), genes vital for GABAergic neurotransmission, as well as cacophony (cac) and paralytic (para), voltage-gated ion channels central to neuronal excitability. To demonstrate the utility of this approach, we manipulated cac in a specific visual interneuron type and discovered differential regulation of calcium signals across subcellular compartments. Thus, FlpStop will facilitate investigations into the interactions between genes, circuits, and computation."xsd:string |
| http://purl.uniprot.org/citations/28211790 | http://purl.org/dc/terms/identifier | "doi:10.7554/elife.22279"xsd:string |
| http://purl.uniprot.org/citations/28211790 | http://purl.uniprot.org/core/author | "Xie M."xsd:string |
| http://purl.uniprot.org/citations/28211790 | http://purl.uniprot.org/core/author | "Gohl D.M."xsd:string |
| http://purl.uniprot.org/citations/28211790 | http://purl.uniprot.org/core/author | "Yang H.H."xsd:string |
| http://purl.uniprot.org/citations/28211790 | http://purl.uniprot.org/core/author | "Clandinin T.R."xsd:string |
| http://purl.uniprot.org/citations/28211790 | http://purl.uniprot.org/core/author | "Isaacman-Beck J."xsd:string |
| http://purl.uniprot.org/citations/28211790 | http://purl.uniprot.org/core/author | "Fisher Y.E."xsd:string |
| http://purl.uniprot.org/citations/28211790 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28211790 | http://purl.uniprot.org/core/name | "Elife"xsd:string |
| http://purl.uniprot.org/citations/28211790 | http://purl.uniprot.org/core/pages | "e22279"xsd:string |
| http://purl.uniprot.org/citations/28211790 | http://purl.uniprot.org/core/title | "FlpStop, a tool for conditional gene control in Drosophila."xsd:string |
| http://purl.uniprot.org/citations/28211790 | http://purl.uniprot.org/core/volume | "6"xsd:string |
| http://purl.uniprot.org/citations/28211790 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28211790 |
| http://purl.uniprot.org/citations/28211790 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/28211790 |
| http://purl.uniprot.org/uniprot/#_E1JGX9-mappedCitation-28211790 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28211790 |
| http://purl.uniprot.org/uniprot/#_E1JGY0-mappedCitation-28211790 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28211790 |
| http://purl.uniprot.org/uniprot/#_A0A4D6K1U1-mappedCitation-28211790 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28211790 |
| http://purl.uniprot.org/uniprot/#_A0A4D6K2Y1-mappedCitation-28211790 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28211790 |
| http://purl.uniprot.org/uniprot/#_A0A4D6K2Y3-mappedCitation-28211790 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28211790 |
| http://purl.uniprot.org/uniprot/#_B7Z0Z2-mappedCitation-28211790 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28211790 |
| http://purl.uniprot.org/uniprot/#_M9MSE4-mappedCitation-28211790 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28211790 |
| http://purl.uniprot.org/uniprot/#_M9MSE6-mappedCitation-28211790 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28211790 |
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