| http://purl.uniprot.org/citations/28214294 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28214294 | http://www.w3.org/2000/01/rdf-schema#comment | "Protein tyrosine kinase 6 (PTK6; also known as Brk) is closely related to the Src family kinases, but lacks a membrane-targeting myristoylation signal. Sublocalization of PTK6 at the plasma membrane enhances its oncogenic potential. To understand the mechanism(s) underlying the oncogenic property of plasma---membrane-associated PTK6, proteins phosphorylated by membrane-targeted myristoylated PTK6 (Myr-PTK6) were enriched and analyzed using a proteomics approach. Eps8 which was identified by this method is phosphorylated by Myr-PTK6 in HEK293 cells. Mouse Eps8 expressed in HEK293 cells is phosphorylated by Myr-PTK6 at residues Tyr497, Tyr524, and Tyr534. Compared to wild-type Eps8 (Eps8 WT), the phosphorylation-defective 3YF mutant (Eps8 3YF) reverts the increased proliferation, migration, and phosphorylation of ERK and FAK mediated by Eps8 WT in HEK293 cells overexpressing PTK6. PTK6 knockdown in T-47D breast cancer cells decreased EGF-induced phosphorylation of Eps8. Endogenous PTK6 phosphorylates ectopically expressed Eps8 WT, but not Eps8 3YF mutant, in EGF-stimulated T-47D cells. The EGF-induced Eps8 phosphorylation enhances activation of ERK and FAK, cell adhesion, and anchorage-independent colony formation in T-47D cells, but not in the PTK6-knokdown T-47D cells. These results indicate that plasma-membrane-associated PTK6 phosphorylates Eps8, which promotes cell proliferation, adhesion, and migration and, thus, tumorigenesis. J. Cell. Biochem. 118: 2887-2895, 2017. © 2017 Wiley Periodicals, Inc."xsd:string |
| http://purl.uniprot.org/citations/28214294 | http://purl.org/dc/terms/identifier | "doi:10.1002/jcb.25939"xsd:string |
| http://purl.uniprot.org/citations/28214294 | http://purl.uniprot.org/core/author | "Lee S.T."xsd:string |
| http://purl.uniprot.org/citations/28214294 | http://purl.uniprot.org/core/author | "Lee Y.H."xsd:string |
| http://purl.uniprot.org/citations/28214294 | http://purl.uniprot.org/core/author | "Park J.S."xsd:string |
| http://purl.uniprot.org/citations/28214294 | http://purl.uniprot.org/core/author | "Ahn S.Y."xsd:string |
| http://purl.uniprot.org/citations/28214294 | http://purl.uniprot.org/core/author | "Shim H.J."xsd:string |
| http://purl.uniprot.org/citations/28214294 | http://purl.uniprot.org/core/author | "Shin W.S."xsd:string |
| http://purl.uniprot.org/citations/28214294 | http://purl.uniprot.org/core/author | "Pyo M."xsd:string |
| http://purl.uniprot.org/citations/28214294 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28214294 | http://purl.uniprot.org/core/name | "J Cell Biochem"xsd:string |
| http://purl.uniprot.org/citations/28214294 | http://purl.uniprot.org/core/pages | "2887-2895"xsd:string |
| http://purl.uniprot.org/citations/28214294 | http://purl.uniprot.org/core/title | "PTK6 Localized at the Plasma Membrane Promotes Cell Proliferation and MigratiOn Through Phosphorylation of Eps8."xsd:string |
| http://purl.uniprot.org/citations/28214294 | http://purl.uniprot.org/core/volume | "118"xsd:string |
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