| http://purl.uniprot.org/citations/2824147 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2824147 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2824147 | http://www.w3.org/2000/01/rdf-schema#comment | "Human tissue-type plasminogen activator (t-PA) cDNA was cloned from uterine tissue and engineered in expression vectors for production in mouse C127 cells. The vectors consisted of the bovine papilloma virus-1 (BPV-1) genome and t-PA transcriptional unit with a mouse metallothionein (MT-1) promoter at the 5' end and MT-1 genomic sequences or SV40 early introns and polyadenylation signals at the 3' end. Analysis of the expression vectors transfected into cells revealed that t-PA is expressed 100-to 200-fold more with an intronless vector utilizing the SV40 polyadenylation signal than with other, intron-containing vectors. RNA analysis of stable cell lines indicated that t-PA expression levels correlated with mRNA abundance. DNA copy number and transcriptional rate of the MT-1 promoter remained constant in cell lines transformed by different BPV expression vectors. Uterine t-PA produced by recombinant DNA means was enzymatically active and similar in properties to Bowes melanoma t-PA."xsd:string |
| http://purl.uniprot.org/citations/2824147 | http://purl.org/dc/terms/identifier | "doi:10.1089/dna.1987.6.461"xsd:string |
| http://purl.uniprot.org/citations/2824147 | http://purl.org/dc/terms/identifier | "doi:10.1089/dna.1987.6.461"xsd:string |
| http://purl.uniprot.org/citations/2824147 | http://purl.uniprot.org/core/author | "Reddy V.B."xsd:string |
| http://purl.uniprot.org/citations/2824147 | http://purl.uniprot.org/core/author | "Reddy V.B."xsd:string |
| http://purl.uniprot.org/citations/2824147 | http://purl.uniprot.org/core/author | "Watkins P."xsd:string |
| http://purl.uniprot.org/citations/2824147 | http://purl.uniprot.org/core/author | "Watkins P."xsd:string |
| http://purl.uniprot.org/citations/2824147 | http://purl.uniprot.org/core/author | "Galli J."xsd:string |
| http://purl.uniprot.org/citations/2824147 | http://purl.uniprot.org/core/author | "Galli J."xsd:string |
| http://purl.uniprot.org/citations/2824147 | http://purl.uniprot.org/core/author | "Garramone A.J."xsd:string |
| http://purl.uniprot.org/citations/2824147 | http://purl.uniprot.org/core/author | "Garramone A.J."xsd:string |
| http://purl.uniprot.org/citations/2824147 | http://purl.uniprot.org/core/author | "Hsiung N."xsd:string |
| http://purl.uniprot.org/citations/2824147 | http://purl.uniprot.org/core/author | "Hsiung N."xsd:string |
| http://purl.uniprot.org/citations/2824147 | http://purl.uniprot.org/core/author | "Sasak H."xsd:string |
| http://purl.uniprot.org/citations/2824147 | http://purl.uniprot.org/core/author | "Sasak H."xsd:string |
| http://purl.uniprot.org/citations/2824147 | http://purl.uniprot.org/core/author | "Wei C.-M."xsd:string |
| http://purl.uniprot.org/citations/2824147 | http://purl.uniprot.org/core/author | "Wei C.-M."xsd:string |
| http://purl.uniprot.org/citations/2824147 | http://purl.uniprot.org/core/date | "1987"xsd:gYear |
| http://purl.uniprot.org/citations/2824147 | http://purl.uniprot.org/core/date | "1987"xsd:gYear |
| http://purl.uniprot.org/citations/2824147 | http://purl.uniprot.org/core/name | "DNA"xsd:string |
| http://purl.uniprot.org/citations/2824147 | http://purl.uniprot.org/core/name | "DNA"xsd:string |
| http://purl.uniprot.org/citations/2824147 | http://purl.uniprot.org/core/pages | "461-472"xsd:string |
| http://purl.uniprot.org/citations/2824147 | http://purl.uniprot.org/core/pages | "461-472"xsd:string |