| http://purl.uniprot.org/citations/28245920 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28245920 | http://www.w3.org/2000/01/rdf-schema#comment | "How metabolism is rewired during embryonic development is still largely unknown, as it remains a major technical challenge to resolve metabolic activities or metabolite levels with spatiotemporal resolution. Here, we investigated metabolic changes during development of organogenesis-stage mouse embryos, focusing on the presomitic mesoderm (PSM). We measured glycolytic labeling kinetics from 13C-glucose tracing experiments and detected elevated glycolysis in the posterior, more undifferentiated PSM. We found evidence that the spatial metabolic differences are functionally relevant during PSM development. To enable real-time quantification of a glycolytic metabolite with spatiotemporal resolution, we generated a pyruvate FRET-sensor reporter mouse line. We revealed dynamic changes in cytosolic pyruvate levels as cells transit toward a more anterior PSM state. Combined, our approach identifies a gradient of glycolytic activity across the PSM, and we provide evidence that these spatiotemporal metabolic changes are intrinsically linked to PSM development and differentiation."xsd:string |
| http://purl.uniprot.org/citations/28245920 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.devcel.2017.01.015"xsd:string |
| http://purl.uniprot.org/citations/28245920 | http://purl.uniprot.org/core/author | "Sauer U."xsd:string |
| http://purl.uniprot.org/citations/28245920 | http://purl.uniprot.org/core/author | "Schultz C."xsd:string |
| http://purl.uniprot.org/citations/28245920 | http://purl.uniprot.org/core/author | "Kuehne A."xsd:string |
| http://purl.uniprot.org/citations/28245920 | http://purl.uniprot.org/core/author | "Kress J."xsd:string |
| http://purl.uniprot.org/citations/28245920 | http://purl.uniprot.org/core/author | "Aulehla A."xsd:string |
| http://purl.uniprot.org/citations/28245920 | http://purl.uniprot.org/core/author | "Stein F."xsd:string |
| http://purl.uniprot.org/citations/28245920 | http://purl.uniprot.org/core/author | "Bulusu V."xsd:string |
| http://purl.uniprot.org/citations/28245920 | http://purl.uniprot.org/core/author | "Snaebjornsson M.T."xsd:string |
| http://purl.uniprot.org/citations/28245920 | http://purl.uniprot.org/core/author | "Prior N."xsd:string |
| http://purl.uniprot.org/citations/28245920 | http://purl.uniprot.org/core/author | "Sonnen K.F."xsd:string |
| http://purl.uniprot.org/citations/28245920 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28245920 | http://purl.uniprot.org/core/name | "Dev Cell"xsd:string |
| http://purl.uniprot.org/citations/28245920 | http://purl.uniprot.org/core/pages | "331-341.e4"xsd:string |
| http://purl.uniprot.org/citations/28245920 | http://purl.uniprot.org/core/title | "Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development."xsd:string |
| http://purl.uniprot.org/citations/28245920 | http://purl.uniprot.org/core/volume | "40"xsd:string |
| http://purl.uniprot.org/citations/28245920 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28245920 |
| http://purl.uniprot.org/citations/28245920 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/28245920 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JD99-mappedCitation-28245920 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28245920 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JDD0-mappedCitation-28245920 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28245920 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JG04-mappedCitation-28245920 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28245920 |
| http://purl.uniprot.org/uniprot/#_A0A087WPT4-mappedCitation-28245920 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28245920 |
| http://purl.uniprot.org/uniprot/#_A0A087WQM3-mappedCitation-28245920 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28245920 |