| http://purl.uniprot.org/citations/2824781 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2824781 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2824781 | http://www.w3.org/2000/01/rdf-schema#comment | "Shigella sonnei contains repetitive sequences, including an insertion element IS1, which can be isolated as double-stranded DNA fragments by DNA denaturation and renaturation and by treatment with S1 nuclease. In this paper, we describe a method of cloning the IS1 fragments prepared by the S1 nuclease digestion technique into phage M13mp8 RFI DNA. Several clones contained IS1, usually with a few additional bases. We isolated and characterized five other repetitive sequences using this method. One sequence, 1264 base-pairs in length, had terminal inverted repeats and contained two open reading frames. This sequence, called IS600, showed about 44% sequence homology with IS3 and was repeated more than 20 times in the Sh. sonnei chromosome. Another sequence (named IS629, 1310 base-pairs in length), which was repeated six times, was found also to be related to IS3 and thus IS600. Two other sequences (named IS630 and IS640, 1159 and 1092 base-pairs in length, respectively), which were repeated approximately ten times, had characteristic terminal inverted repeats and contained a large open reading frame coding for a protein. The inverted repeat sequences of IS630 were similar to the sequence at one end of IS200, a Salmonella-specific IS element. The fifth sequence, repeated ten times in Sh. sonnei, had about 98% sequence homology with a portion of IS2. The method described here can be applied to the isolation of IS or iso-IS elements present in any other bacterial chromosome."xsd:string |
| http://purl.uniprot.org/citations/2824781 | http://purl.org/dc/terms/identifier | "doi:10.1016/0022-2836(87)90023-4"xsd:string |
| http://purl.uniprot.org/citations/2824781 | http://purl.org/dc/terms/identifier | "doi:10.1016/0022-2836(87)90023-4"xsd:string |
| http://purl.uniprot.org/citations/2824781 | http://purl.uniprot.org/core/author | "Ohtsubo E."xsd:string |
| http://purl.uniprot.org/citations/2824781 | http://purl.uniprot.org/core/author | "Ohtsubo E."xsd:string |
| http://purl.uniprot.org/citations/2824781 | http://purl.uniprot.org/core/author | "Maeda Y."xsd:string |
| http://purl.uniprot.org/citations/2824781 | http://purl.uniprot.org/core/author | "Maeda Y."xsd:string |
| http://purl.uniprot.org/citations/2824781 | http://purl.uniprot.org/core/author | "Ohtsubo H."xsd:string |
| http://purl.uniprot.org/citations/2824781 | http://purl.uniprot.org/core/author | "Ohtsubo H."xsd:string |
| http://purl.uniprot.org/citations/2824781 | http://purl.uniprot.org/core/author | "Matsutani S."xsd:string |
| http://purl.uniprot.org/citations/2824781 | http://purl.uniprot.org/core/author | "Matsutani S."xsd:string |
| http://purl.uniprot.org/citations/2824781 | http://purl.uniprot.org/core/date | "1987"xsd:gYear |
| http://purl.uniprot.org/citations/2824781 | http://purl.uniprot.org/core/date | "1987"xsd:gYear |
| http://purl.uniprot.org/citations/2824781 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/2824781 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/2824781 | http://purl.uniprot.org/core/pages | "445-455"xsd:string |
| http://purl.uniprot.org/citations/2824781 | http://purl.uniprot.org/core/pages | "445-455"xsd:string |
| http://purl.uniprot.org/citations/2824781 | http://purl.uniprot.org/core/title | "Isolation and characterization of IS elements repeated in the bacterial chromosome."xsd:string |
| http://purl.uniprot.org/citations/2824781 | http://purl.uniprot.org/core/title | "Isolation and characterization of IS elements repeated in the bacterial chromosome."xsd:string |
| http://purl.uniprot.org/citations/2824781 | http://purl.uniprot.org/core/volume | "196"xsd:string |
| http://purl.uniprot.org/citations/2824781 | http://purl.uniprot.org/core/volume | "196"xsd:string |
| http://purl.uniprot.org/citations/2824781 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2824781 |
| http://purl.uniprot.org/citations/2824781 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2824781 |