| http://purl.uniprot.org/citations/28270562 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28270562 | http://www.w3.org/2000/01/rdf-schema#comment | "Sperm-specific phospholipase C zeta (PLCζ) is widely considered to be the physiological stimulus that evokes intracellular calcium (Ca2+) oscillations that are essential for the initiation of egg activation during mammalian fertilisation. A recent genetic study reported a male infertility case that was directly associated with a point mutation in the PLCζ C2 domain, where an isoleucine residue had been substituted with a phenylalanine (I489F). Here, we have analysed the effect of this mutation on the in vivo Ca2+ oscillation-inducing activity and the in vitro biochemical properties of human PLCζ. Microinjection of cRNA or recombinant protein corresponding to PLCζI489F mutant at physiological concentrations completely failed to cause Ca2+ oscillations and trigger development. However, this infertile phenotype could be effectively rescued by microinjection of relatively high (non-physiological) amounts of recombinant mutant PLCζI489F protein, leading to Ca2+ oscillations and egg activation. Our in vitro biochemical analysis suggested that the PLCζI489F mutant displayed similar enzymatic properties, but dramatically reduced binding to PI(3)P and PI(5)P-containing liposomes compared with wild-type PLCζ. Our findings highlight the importance of PLCζ at fertilisation and the vital role of the C2 domain in PLCζ function, possibly due to its novel binding characteristics."xsd:string |
| http://purl.uniprot.org/citations/28270562 | http://purl.org/dc/terms/identifier | "doi:10.1042/bcj20161057"xsd:string |
| http://purl.uniprot.org/citations/28270562 | http://purl.uniprot.org/core/author | "Lai F.A."xsd:string |
| http://purl.uniprot.org/citations/28270562 | http://purl.uniprot.org/core/author | "Swann K."xsd:string |
| http://purl.uniprot.org/citations/28270562 | http://purl.uniprot.org/core/author | "Beck K."xsd:string |
| http://purl.uniprot.org/citations/28270562 | http://purl.uniprot.org/core/author | "Buntwal L."xsd:string |
| http://purl.uniprot.org/citations/28270562 | http://purl.uniprot.org/core/author | "Calver B.L."xsd:string |
| http://purl.uniprot.org/citations/28270562 | http://purl.uniprot.org/core/author | "Nomikos M."xsd:string |
| http://purl.uniprot.org/citations/28270562 | http://purl.uniprot.org/core/author | "Sanders J.R."xsd:string |
| http://purl.uniprot.org/citations/28270562 | http://purl.uniprot.org/core/author | "Lofty M."xsd:string |
| http://purl.uniprot.org/citations/28270562 | http://purl.uniprot.org/core/author | "Sideratou Z."xsd:string |
| http://purl.uniprot.org/citations/28270562 | http://purl.uniprot.org/core/author | "Stamatiadis P."xsd:string |
| http://purl.uniprot.org/citations/28270562 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28270562 | http://purl.uniprot.org/core/name | "Biochem J"xsd:string |
| http://purl.uniprot.org/citations/28270562 | http://purl.uniprot.org/core/pages | "1003-1016"xsd:string |
| http://purl.uniprot.org/citations/28270562 | http://purl.uniprot.org/core/title | "Male infertility-linked point mutation reveals a vital binding role for the C2 domain of sperm PLCzeta."xsd:string |
| http://purl.uniprot.org/citations/28270562 | http://purl.uniprot.org/core/volume | "474"xsd:string |
| http://purl.uniprot.org/citations/28270562 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28270562 |
| http://purl.uniprot.org/citations/28270562 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/28270562 |
| http://purl.uniprot.org/uniprot/Q86YW0#attribution-02C377C997499E7565FEB87813F57332 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/28270562 |
| http://purl.uniprot.org/uniprot/Q86YW0#attribution-6B653D9DA8BD20A92202A15E92D39988 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/28270562 |
| http://purl.uniprot.org/uniprot/#_A0A140VJR9-mappedCitation-28270562 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28270562 |
| http://purl.uniprot.org/uniprot/#_Q86YW0-mappedCitation-28270562 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28270562 |
| http://purl.uniprot.org/uniprot/#_Q8N7S5-mappedCitation-28270562 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28270562 |