| http://purl.uniprot.org/citations/28294942 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28294942 | http://www.w3.org/2000/01/rdf-schema#comment | "Pentameric ligand-gated ion channels (pLGICs) mediate fast chemical signaling through global allosteric transitions. Despite the existence of several high-resolution structures of pLGICs, their dynamical properties remain elusive. Using the proton-gated channel GLIC, we engineered multiple fluorescent reporters, each incorporating a bimane and a tryptophan/tyrosine, whose close distance causes fluorescence quenching. We show that proton application causes a global compaction of the extracellular subunit interface, coupled to an outward motion of the M2-M3 loop near the channel gate. These movements are highly similar in lipid vesicles and detergent micelles. These reorganizations are essentially completed within 2 ms and occur without channel opening at low proton concentration, indicating that they report a pre-active intermediate state in the transition pathway toward activation. This provides a template to investigate the gating of eukaryotic neurotransmitter receptors, for which intermediate states also participate in activation."xsd:string |
| http://purl.uniprot.org/citations/28294942 | http://purl.org/dc/terms/identifier | "doi:10.7554/elife.23955"xsd:string |
| http://purl.uniprot.org/citations/28294942 | http://purl.uniprot.org/core/author | "Delarue M."xsd:string |
| http://purl.uniprot.org/citations/28294942 | http://purl.uniprot.org/core/author | "Schmidpeter P.A."xsd:string |
| http://purl.uniprot.org/citations/28294942 | http://purl.uniprot.org/core/author | "Corringer P.J."xsd:string |
| http://purl.uniprot.org/citations/28294942 | http://purl.uniprot.org/core/author | "Edelstein S.J."xsd:string |
| http://purl.uniprot.org/citations/28294942 | http://purl.uniprot.org/core/author | "Joseph D."xsd:string |
| http://purl.uniprot.org/citations/28294942 | http://purl.uniprot.org/core/author | "Nimigean C.M."xsd:string |
| http://purl.uniprot.org/citations/28294942 | http://purl.uniprot.org/core/author | "Fourati Z."xsd:string |
| http://purl.uniprot.org/citations/28294942 | http://purl.uniprot.org/core/author | "Menny A."xsd:string |
| http://purl.uniprot.org/citations/28294942 | http://purl.uniprot.org/core/author | "Drege E."xsd:string |
| http://purl.uniprot.org/citations/28294942 | http://purl.uniprot.org/core/author | "Lefebvre S.N."xsd:string |
| http://purl.uniprot.org/citations/28294942 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28294942 | http://purl.uniprot.org/core/name | "Elife"xsd:string |
| http://purl.uniprot.org/citations/28294942 | http://purl.uniprot.org/core/pages | "e23955"xsd:string |
| http://purl.uniprot.org/citations/28294942 | http://purl.uniprot.org/core/title | "Identification of a pre-active conformation of a pentameric channel receptor."xsd:string |
| http://purl.uniprot.org/citations/28294942 | http://purl.uniprot.org/core/volume | "6"xsd:string |
| http://purl.uniprot.org/citations/28294942 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28294942 |
| http://purl.uniprot.org/citations/28294942 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/28294942 |
| http://purl.uniprot.org/uniprot/#_Q7NDN8-mappedCitation-28294942 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28294942 |
| http://purl.uniprot.org/uniprot/Q7NDN8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/28294942 |