| http://purl.uniprot.org/citations/2829709 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2829709 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2829709 | http://www.w3.org/2000/01/rdf-schema#comment | "To identify the nucleotide changes that occur in drug-induced thymidine kinase (TK) mutants of herpes simplex virus type 2 (HSV-2), we compared the nucleotide sequences of the tk genes of two mutant HSV-2 clones isolated from a patient who had been treated with acyclovir [9-(2-hydroxyethoxymethyl)guanine; ACV] with the nucleotide sequence of the parental TK+ HSV-2(8703) strain isolated from the same patient. One of the mutants, TK-altered (TKA) HSV-2(9637), was ACV resistant but induced the incorporation of [14C]thymidine into the DNA of infected rabbit skin cells. The nucleotide sequence of the tk gene of mutant TKA HSV-2(9637) had a single change (G to A) at nucleotide 668, which would cause an arginine-to-histidine substitution at amino acid residue 223 of the TK polypeptide. The second ACV-resistant mutant, TK-HSV-2(8710), did not induce detectable incorporation of [14C]thymidine into the DNA of infected rabbit skin cells. This mutant exhibited a deletion of a single base at nucleotide 217 of its nucleotide sequence. This deletion would cause a frameshift mutation at amino acid residue 73 and chain termination at amino acid residue 86 of the TK polypeptide. The nucleotide sequence of TK+ HSV-2(8703) was the same as that of the laboratory strain, TK+ HSV-2(333). The nucleotide sequence of a bromodeoxyuridine-resistant TK-HSV-2(333) mutant of TK+ HSV-2(333) also exhibited a single-base deletion, but at nucleotide 439. This deletion would cause a frameshift mutation at amino acid residue 147 and chain termination at amino acid residue 182. The frameshift mutations of TK- HSV(8710) and TK-HSV-2(333), respectively, occurred in sequences in which C was repeated three times and G was repeated seven times. The results raise the possibility that TK-frameshift mutations of HSV-2 may be common."xsd:string |
| http://purl.uniprot.org/citations/2829709 | http://purl.org/dc/terms/identifier | "doi:10.1128/aac.31.10.1483"xsd:string |
| http://purl.uniprot.org/citations/2829709 | http://purl.org/dc/terms/identifier | "doi:10.1128/aac.31.10.1483"xsd:string |
| http://purl.uniprot.org/citations/2829709 | http://purl.uniprot.org/core/author | "Ichimura H."xsd:string |
| http://purl.uniprot.org/citations/2829709 | http://purl.uniprot.org/core/author | "Ichimura H."xsd:string |
| http://purl.uniprot.org/citations/2829709 | http://purl.uniprot.org/core/author | "Sheppard M."xsd:string |
| http://purl.uniprot.org/citations/2829709 | http://purl.uniprot.org/core/author | "Sheppard M."xsd:string |
| http://purl.uniprot.org/citations/2829709 | http://purl.uniprot.org/core/author | "Otsuka H."xsd:string |
| http://purl.uniprot.org/citations/2829709 | http://purl.uniprot.org/core/author | "Otsuka H."xsd:string |
| http://purl.uniprot.org/citations/2829709 | http://purl.uniprot.org/core/author | "Ellis M.N."xsd:string |
| http://purl.uniprot.org/citations/2829709 | http://purl.uniprot.org/core/author | "Ellis M.N."xsd:string |
| http://purl.uniprot.org/citations/2829709 | http://purl.uniprot.org/core/author | "Fyfe J.A."xsd:string |
| http://purl.uniprot.org/citations/2829709 | http://purl.uniprot.org/core/author | "Fyfe J.A."xsd:string |
| http://purl.uniprot.org/citations/2829709 | http://purl.uniprot.org/core/author | "Kit S."xsd:string |
| http://purl.uniprot.org/citations/2829709 | http://purl.uniprot.org/core/author | "Kit S."xsd:string |
| http://purl.uniprot.org/citations/2829709 | http://purl.uniprot.org/core/author | "Nusinoff-Lehrman S."xsd:string |
| http://purl.uniprot.org/citations/2829709 | http://purl.uniprot.org/core/author | "Nusinoff-Lehrman S."xsd:string |
| http://purl.uniprot.org/citations/2829709 | http://purl.uniprot.org/core/date | "1987"xsd:gYear |
| http://purl.uniprot.org/citations/2829709 | http://purl.uniprot.org/core/date | "1987"xsd:gYear |
| http://purl.uniprot.org/citations/2829709 | http://purl.uniprot.org/core/name | "Antimicrob. Agents Chemother."xsd:string |
| http://purl.uniprot.org/citations/2829709 | http://purl.uniprot.org/core/name | "Antimicrob. Agents Chemother."xsd:string |
| http://purl.uniprot.org/citations/2829709 | http://purl.uniprot.org/core/pages | "1483-1490"xsd:string |
| http://purl.uniprot.org/citations/2829709 | http://purl.uniprot.org/core/pages | "1483-1490"xsd:string |