http://purl.uniprot.org/citations/2830166 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/2830166 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/2830166 | http://www.w3.org/2000/01/rdf-schema#comment | "The expression and secretion of Bacillus amyloliquefaciens alpha-amylase was studied in yeast Saccharomyces cerevisiae. The Bacillus promoter was removed by BAL 31 digestion and three forms of the alpha-amylase gene were constructed: the Bacillus signal sequence was either complete (YEp alpha a1), partial (YEp alpha a2) or missing (YEp alpha a3). Secretion of alpha-amylase into the culture medium was obtained with the complete signal sequence only. The secreted alpha-amylase was glycosylated and its signal peptide was apparently processed. The glycosylated alpha-amylase remained active. The enzyme produced by the other constructions was not glycosylated and thus probably remained in the cytoplasm."xsd:string |
http://purl.uniprot.org/citations/2830166 | http://purl.org/dc/terms/identifier | "doi:10.1016/0378-1119(87)90324-6"xsd:string |
http://purl.uniprot.org/citations/2830166 | http://purl.org/dc/terms/identifier | "doi:10.1016/0378-1119(87)90324-6"xsd:string |
http://purl.uniprot.org/citations/2830166 | http://purl.uniprot.org/core/author | "Hackman P."xsd:string |
http://purl.uniprot.org/citations/2830166 | http://purl.uniprot.org/core/author | "Hackman P."xsd:string |
http://purl.uniprot.org/citations/2830166 | http://purl.uniprot.org/core/author | "Karaenen S."xsd:string |
http://purl.uniprot.org/citations/2830166 | http://purl.uniprot.org/core/author | "Karaenen S."xsd:string |
http://purl.uniprot.org/citations/2830166 | http://purl.uniprot.org/core/author | "Knowles J.K.C."xsd:string |
http://purl.uniprot.org/citations/2830166 | http://purl.uniprot.org/core/author | "Knowles J.K.C."xsd:string |
http://purl.uniprot.org/citations/2830166 | http://purl.uniprot.org/core/author | "Lehtovaara P."xsd:string |
http://purl.uniprot.org/citations/2830166 | http://purl.uniprot.org/core/author | "Lehtovaara P."xsd:string |
http://purl.uniprot.org/citations/2830166 | http://purl.uniprot.org/core/author | "Ruohonen L."xsd:string |
http://purl.uniprot.org/citations/2830166 | http://purl.uniprot.org/core/author | "Ruohonen L."xsd:string |
http://purl.uniprot.org/citations/2830166 | http://purl.uniprot.org/core/date | "1987"xsd:gYear |
http://purl.uniprot.org/citations/2830166 | http://purl.uniprot.org/core/date | "1987"xsd:gYear |
http://purl.uniprot.org/citations/2830166 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
http://purl.uniprot.org/citations/2830166 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
http://purl.uniprot.org/citations/2830166 | http://purl.uniprot.org/core/pages | "161-170"xsd:string |
http://purl.uniprot.org/citations/2830166 | http://purl.uniprot.org/core/pages | "161-170"xsd:string |
http://purl.uniprot.org/citations/2830166 | http://purl.uniprot.org/core/title | "Efficient secretion of Bacillus amyloliquefaciens alpha-amylase by its own signal peptide from Saccharomyces cerevisiae host cells."xsd:string |
http://purl.uniprot.org/citations/2830166 | http://purl.uniprot.org/core/title | "Efficient secretion of Bacillus amyloliquefaciens alpha-amylase by its own signal peptide from Saccharomyces cerevisiae host cells."xsd:string |
http://purl.uniprot.org/citations/2830166 | http://purl.uniprot.org/core/volume | "59"xsd:string |
http://purl.uniprot.org/citations/2830166 | http://purl.uniprot.org/core/volume | "59"xsd:string |