| http://purl.uniprot.org/citations/28317950 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28317950 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28317950 | http://www.w3.org/2000/01/rdf-schema#comment | "Ecotoxicological studies have indicated the reprotoxicity of uranium (U) in zebrafish, but its molecular mechanisms remain unclear. Due to the non-covalent nature of U-protein complexes, canonical proteomics approaches are often not relevant as they usually use denaturating reagents or solvents. In this study, non-denaturating (ND) methods were used to obtain insight into the nature of U potential targets in ovaries of reproduced and non-reproduced zebrafish after 20 days of exposure to an environmentally relevant U concentration (20 μg L-1). After the ND sample preparation, 1-dimensional (SEC) and 2-dimensional (OGE × SEC) separations followed by ICP-sector-field MS measurements (U, P, Fe, Cu, and Zn) enabled the determination of chemical characteristics (MW, pI) of the metal-protein complexes. Phosphorus and U coelution confirmed the affinity of U for P-containing proteins. In addition, 2D separation allowed the discrimination of Fe-metalloproteins as potential U targets. Finally, 20 protein candidates for U complexation were identified after tryptic digestion conditions by LC-ESI FT MS and a database search. Potential U targets were mainly involved in three biological processes: oxidative stress regulation (SOD, GST), cytoskeleton structure (actin) and embryo early development (vtg, initiation factor)."xsd:string |
| http://purl.uniprot.org/citations/28317950 | http://purl.org/dc/terms/identifier | "doi:10.1039/c6mt00291a"xsd:string |
| http://purl.uniprot.org/citations/28317950 | http://purl.uniprot.org/core/author | "Simon O."xsd:string |
| http://purl.uniprot.org/citations/28317950 | http://purl.uniprot.org/core/author | "Simon O."xsd:string |
| http://purl.uniprot.org/citations/28317950 | http://purl.uniprot.org/core/author | "Lobinski R."xsd:string |
| http://purl.uniprot.org/citations/28317950 | http://purl.uniprot.org/core/author | "Lobinski R."xsd:string |
| http://purl.uniprot.org/citations/28317950 | http://purl.uniprot.org/core/author | "Arnaudguilhem C."xsd:string |
| http://purl.uniprot.org/citations/28317950 | http://purl.uniprot.org/core/author | "Arnaudguilhem C."xsd:string |
| http://purl.uniprot.org/citations/28317950 | http://purl.uniprot.org/core/author | "Eb-Levadoux Y."xsd:string |
| http://purl.uniprot.org/citations/28317950 | http://purl.uniprot.org/core/author | "Eb-Levadoux Y."xsd:string |
| http://purl.uniprot.org/citations/28317950 | http://purl.uniprot.org/core/author | "Frelon S."xsd:string |
| http://purl.uniprot.org/citations/28317950 | http://purl.uniprot.org/core/author | "Frelon S."xsd:string |
| http://purl.uniprot.org/citations/28317950 | http://purl.uniprot.org/core/author | "Mounicou S."xsd:string |
| http://purl.uniprot.org/citations/28317950 | http://purl.uniprot.org/core/author | "Mounicou S."xsd:string |
| http://purl.uniprot.org/citations/28317950 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28317950 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28317950 | http://purl.uniprot.org/core/name | "Metallomics"xsd:string |
| http://purl.uniprot.org/citations/28317950 | http://purl.uniprot.org/core/name | "Metallomics"xsd:string |
| http://purl.uniprot.org/citations/28317950 | http://purl.uniprot.org/core/pages | "525-534"xsd:string |
| http://purl.uniprot.org/citations/28317950 | http://purl.uniprot.org/core/pages | "525-534"xsd:string |
| http://purl.uniprot.org/citations/28317950 | http://purl.uniprot.org/core/title | "In vivo identification of potential uranium protein targets in zebrafish ovaries after chronic waterborne exposure."xsd:string |
| http://purl.uniprot.org/citations/28317950 | http://purl.uniprot.org/core/title | "In vivo identification of potential uranium protein targets in zebrafish ovaries after chronic waterborne exposure."xsd:string |
| http://purl.uniprot.org/citations/28317950 | http://purl.uniprot.org/core/volume | "9"xsd:string |