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http://purl.uniprot.org/citations/2833753http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/2833753http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/2833753http://www.w3.org/2000/01/rdf-schema#comment"The cell-surface glycoprotein Mo1 is a member of the family of leukocyte cell adhesion molecules (Leu-CAMs) that includes lymphocyte function-associated antigen 1 (LFA-1) and p150,95. Each Leu-CAM is a heterodimer with a distinct alpha subunit noncovalently associated with a common beta subunit. Leu-CAMs play crucial roles in cell-cell and cell-matrix interactions. We describe the isolation and analysis of two partial cDNA clones encoding the alpha subunit of the Leu-CAM Mo1 in humans and guinea pigs. A monoclonal antibody directed against an epitope in the carboxyl-terminal portion of the guinea pig alpha chain was used for immunoscreening a lambda gt11 expression library. The sequence of a 378-base-pair insert from one immunoreactive clone revealed a single continuous open reading frame encoding 126 amino acids including a 26-amino acid tryptic peptide isolated from the purified guinea pig alpha subunit. A cDNA clone of identical size was isolated from a human monocyte/lymphocyte cDNA library by using the guinea pig clone as a probe. The human clone also encoded a 126-amino acid peptide including the sequence of an additional tryptic peptide present in purified human Mo1 alpha chain. RNA gel blots revealed that mature Mo1 alpha chain mRNA is approximately 5 kilobases in guinea pigs and humans. Southern analysis of DNA from hamster-human hybrids localized the human Mo1 alpha chain to chromosome 16, which has been shown to contain the gene for the alpha chain of lymphocyte function-associated antigen 1. A comparison of the Mo1 alpha chain coding region revealed significant homologies with carboxyl-terminal portions of the alpha subunits of fibronectin, vitronectin, and platelet IIb/IIIa receptors. These data suggest that the alpha subunits of Leu-CAMs evolved by gene duplication from a common ancestral gene and strengthen the hypothesis that the alpha subunits of these heterodimeric cell adhesion molecules on myeloid and lymphoid cells, platelets, and fibroblasts are evolutionary related."xsd:string
http://purl.uniprot.org/citations/2833753http://purl.org/dc/terms/identifier"doi:10.1073/pnas.85.8.2776"xsd:string
http://purl.uniprot.org/citations/2833753http://purl.org/dc/terms/identifier"doi:10.1073/pnas.85.8.2776"xsd:string
http://purl.uniprot.org/citations/2833753http://purl.uniprot.org/core/author"Harris P."xsd:string
http://purl.uniprot.org/citations/2833753http://purl.uniprot.org/core/author"Harris P."xsd:string
http://purl.uniprot.org/citations/2833753http://purl.uniprot.org/core/author"Tenen D.G."xsd:string
http://purl.uniprot.org/citations/2833753http://purl.uniprot.org/core/author"Tenen D.G."xsd:string
http://purl.uniprot.org/citations/2833753http://purl.uniprot.org/core/author"Arnaout M.A."xsd:string
http://purl.uniprot.org/citations/2833753http://purl.uniprot.org/core/author"Arnaout M.A."xsd:string
http://purl.uniprot.org/citations/2833753http://purl.uniprot.org/core/author"Remold-O'Donnell E."xsd:string
http://purl.uniprot.org/citations/2833753http://purl.uniprot.org/core/author"Remold-O'Donnell E."xsd:string
http://purl.uniprot.org/citations/2833753http://purl.uniprot.org/core/author"Pierce M.W."xsd:string
http://purl.uniprot.org/citations/2833753http://purl.uniprot.org/core/author"Pierce M.W."xsd:string
http://purl.uniprot.org/citations/2833753http://purl.uniprot.org/core/date"1988"xsd:gYear
http://purl.uniprot.org/citations/2833753http://purl.uniprot.org/core/date"1988"xsd:gYear
http://purl.uniprot.org/citations/2833753http://purl.uniprot.org/core/name"Proc. Natl. Acad. Sci. U.S.A."xsd:string
http://purl.uniprot.org/citations/2833753http://purl.uniprot.org/core/name"Proc. Natl. Acad. Sci. U.S.A."xsd:string
http://purl.uniprot.org/citations/2833753http://purl.uniprot.org/core/pages"2776-2780"xsd:string
http://purl.uniprot.org/citations/2833753http://purl.uniprot.org/core/pages"2776-2780"xsd:string
http://purl.uniprot.org/citations/2833753http://purl.uniprot.org/core/title"Molecular cloning of the alpha subunit of human and guinea pig leukocyte adhesion glycoprotein Mo1: chromosomal localization and homology to the alpha subunits of integrins."xsd:string
http://purl.uniprot.org/citations/2833753http://purl.uniprot.org/core/title"Molecular cloning of the alpha subunit of human and guinea pig leukocyte adhesion glycoprotein Mo1: chromosomal localization and homology to the alpha subunits of integrins."xsd:string
http://purl.uniprot.org/citations/2833753http://purl.uniprot.org/core/volume"85"xsd:string
http://purl.uniprot.org/citations/2833753http://purl.uniprot.org/core/volume"85"xsd:string
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