| http://purl.uniprot.org/citations/28362257 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28362257 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28362257 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/28362257 | http://www.w3.org/2000/01/rdf-schema#comment | "Strict L-chiral rejection through Gly-cisPro motif during chiral proofreading underlies the inability of D-aminoacyl-tRNA deacylase (DTD) to discriminate between D-amino acids and achiral glycine. The consequent Gly-tRNAGly 'misediting paradox' is resolved by EF-Tu in the cell. Here, we show that DTD's active site architecture can efficiently edit mischarged Gly-tRNAAla species four orders of magnitude more efficiently than even AlaRS, the only ubiquitous cellular checkpoint known for clearing the error. Also, DTD knockout in AlaRS editing-defective background causes pronounced toxicity in Escherichia coli even at low-glycine levels which is alleviated by alanine supplementation. We further demonstrate that DTD positively selects the universally invariant tRNAAla-specific G3•U70. Moreover, DTD's activity on non-cognate Gly-tRNAAla is conserved across all bacteria and eukaryotes, suggesting DTD's key cellular role as a glycine deacylator. Our study thus reveals a hitherto unknown function of DTD in cracking the universal mechanistic dilemma encountered by AlaRS, and its physiological importance."xsd:string |
| http://purl.uniprot.org/citations/28362257 | http://purl.org/dc/terms/identifier | "doi:10.7554/elife.24001"xsd:string |
| http://purl.uniprot.org/citations/28362257 | http://purl.org/dc/terms/identifier | "doi:10.7554/elife.24001"xsd:string |
| http://purl.uniprot.org/citations/28362257 | http://purl.uniprot.org/core/author | "Pawar K.I."xsd:string |
| http://purl.uniprot.org/citations/28362257 | http://purl.uniprot.org/core/author | "Pawar K.I."xsd:string |
| http://purl.uniprot.org/citations/28362257 | http://purl.uniprot.org/core/author | "Kruparani S.P."xsd:string |
| http://purl.uniprot.org/citations/28362257 | http://purl.uniprot.org/core/author | "Kruparani S.P."xsd:string |
| http://purl.uniprot.org/citations/28362257 | http://purl.uniprot.org/core/author | "Kuncha S.K."xsd:string |
| http://purl.uniprot.org/citations/28362257 | http://purl.uniprot.org/core/author | "Kuncha S.K."xsd:string |
| http://purl.uniprot.org/citations/28362257 | http://purl.uniprot.org/core/author | "Routh S.B."xsd:string |
| http://purl.uniprot.org/citations/28362257 | http://purl.uniprot.org/core/author | "Routh S.B."xsd:string |
| http://purl.uniprot.org/citations/28362257 | http://purl.uniprot.org/core/author | "Sankaranarayanan R."xsd:string |
| http://purl.uniprot.org/citations/28362257 | http://purl.uniprot.org/core/author | "Sankaranarayanan R."xsd:string |
| http://purl.uniprot.org/citations/28362257 | http://purl.uniprot.org/core/author | "Seenivasan A."xsd:string |
| http://purl.uniprot.org/citations/28362257 | http://purl.uniprot.org/core/author | "Seenivasan A."xsd:string |
| http://purl.uniprot.org/citations/28362257 | http://purl.uniprot.org/core/author | "Suma K."xsd:string |
| http://purl.uniprot.org/citations/28362257 | http://purl.uniprot.org/core/author | "Suma K."xsd:string |
| http://purl.uniprot.org/citations/28362257 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28362257 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28362257 | http://purl.uniprot.org/core/name | "Elife"xsd:string |
| http://purl.uniprot.org/citations/28362257 | http://purl.uniprot.org/core/name | "Elife"xsd:string |
| http://purl.uniprot.org/citations/28362257 | http://purl.uniprot.org/core/title | "Role of D-aminoacyl-tRNA deacylase beyond chiral proofreading as a cellular defense against glycine mischarging by AlaRS."xsd:string |