| http://purl.uniprot.org/citations/28379186 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28379186 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28379186 | http://www.w3.org/2000/01/rdf-schema#comment | "The PKS-NRPS-derived tetramic acid equisetin and its N-desmethyl derivative trichosetin exhibit remarkable biological activities against a variety of organisms, including plants and bacteria, e.g., Staphylococcus aureus. The equisetin biosynthetic gene cluster was first described in Fusarium heterosporum, a species distantly related to the notorious rice pathogen Fusarium fujikuroi. Here we present the activation and characterization of a homologous, but silent, gene cluster in F. fujikuroi. Bioinformatic analysis revealed that this cluster does not contain the equisetin N-methyltransferase gene eqxD and consequently, trichosetin was isolated as final product. The adaption of the inducible, tetracycline-dependent Tet-on promoter system from Aspergillus niger achieved a controlled overproduction of this toxic metabolite and a functional characterization of each cluster gene in F. fujikuroi. Overexpression of one of the two cluster-specific transcription factor (TF) genes, TF22, led to an activation of the three biosynthetic cluster genes, including the PKS-NRPS key gene. In contrast, overexpression of TF23, encoding a second Zn(II)₂Cys₆ TF, did not activate adjacent cluster genes. Instead, TF23 was induced by the final product trichosetin and was required for expression of the transporter-encoding gene MFS-T. TF23 and MFS-T likely act in consort and contribute to detoxification of trichosetin and therefore, self-protection of the producing fungus."xsd:string |
| http://purl.uniprot.org/citations/28379186 | http://purl.org/dc/terms/identifier | "doi:10.3390/toxins9040126"xsd:string |
| http://purl.uniprot.org/citations/28379186 | http://purl.org/dc/terms/identifier | "doi:10.3390/toxins9040126"xsd:string |
| http://purl.uniprot.org/citations/28379186 | http://purl.uniprot.org/core/author | "Baumann L."xsd:string |
| http://purl.uniprot.org/citations/28379186 | http://purl.uniprot.org/core/author | "Baumann L."xsd:string |
| http://purl.uniprot.org/citations/28379186 | http://purl.uniprot.org/core/author | "Tudzynski B."xsd:string |
| http://purl.uniprot.org/citations/28379186 | http://purl.uniprot.org/core/author | "Tudzynski B."xsd:string |
| http://purl.uniprot.org/citations/28379186 | http://purl.uniprot.org/core/author | "Humpf H.U."xsd:string |
| http://purl.uniprot.org/citations/28379186 | http://purl.uniprot.org/core/author | "Humpf H.U."xsd:string |
| http://purl.uniprot.org/citations/28379186 | http://purl.uniprot.org/core/author | "Janevska S."xsd:string |
| http://purl.uniprot.org/citations/28379186 | http://purl.uniprot.org/core/author | "Janevska S."xsd:string |
| http://purl.uniprot.org/citations/28379186 | http://purl.uniprot.org/core/author | "Arndt B."xsd:string |
| http://purl.uniprot.org/citations/28379186 | http://purl.uniprot.org/core/author | "Arndt B."xsd:string |
| http://purl.uniprot.org/citations/28379186 | http://purl.uniprot.org/core/author | "Apken L.H."xsd:string |
| http://purl.uniprot.org/citations/28379186 | http://purl.uniprot.org/core/author | "Apken L.H."xsd:string |
| http://purl.uniprot.org/citations/28379186 | http://purl.uniprot.org/core/author | "Mauriz Marques L.M."xsd:string |
| http://purl.uniprot.org/citations/28379186 | http://purl.uniprot.org/core/author | "Mauriz Marques L.M."xsd:string |
| http://purl.uniprot.org/citations/28379186 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28379186 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28379186 | http://purl.uniprot.org/core/name | "Toxins"xsd:string |
| http://purl.uniprot.org/citations/28379186 | http://purl.uniprot.org/core/name | "Toxins"xsd:string |
| http://purl.uniprot.org/citations/28379186 | http://purl.uniprot.org/core/title | "Establishment of the inducible Tet-On system for the activation of the silent trichosetin gene cluster in Fusarium fujikuroi."xsd:string |
| http://purl.uniprot.org/citations/28379186 | http://purl.uniprot.org/core/title | "Establishment of the inducible Tet-On system for the activation of the silent trichosetin gene cluster in Fusarium fujikuroi."xsd:string |