| http://purl.uniprot.org/citations/28406939 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28406939 | http://www.w3.org/2000/01/rdf-schema#comment | "The mechanism of the negative regulation of proopiomelanocortin gene (Pomc) by glucocorticoids (Gcs) is still unclear in many points. Here, we demonstrated the involvement of neurogenic differentiation factor 1 (NeuroD1) in the Gc-mediated negative regulation of Pomc. Murine pituitary adrenocorticotropic hormone (ACTH) producing corticotroph tumor-derived AtT20 cells were treated with dexamethasone (DEX) (1-100 nM) and cultured for 24 hrs. Thereafter, Pomc mRNA expression was studied by quantitative real-time PCR and rat Pomc promoter (-703/+58) activity was examined by luciferase assay. Both Pomc mRNA expression and Pomc promoter activity were inhibited by DEX in a dose-dependent manner. Deletion and point mutant analyses of Pomc promoter suggested that the DEX-mediated transcriptional repression was mediated via E-box that exists at -376/-371 in the promoter. Since NeuroD1 is known to bind to and activate E-box of the Pomc promoter, we next examined the effect of DEX on NeuroD1 expression. Interestingly, DEX dose-dependently inhibited NeuroD1 mRNA expression, mouse NeuroD1 promoter (-2.2-kb) activity, and NeuroD1 protein expression in AtT20 cells. In addition, we confirmed the inhibitory effect of DEX on the interaction of NeuroD1 and E-box on Pomc promoter by chromatin immunoprecipitation (ChIP) assay. Finally, overexpression of mouse NeuroD1 could rescue the DEX-mediated inhibition of Pomc mRNA expression and Pomc promoter activity. Taken together, it is suggested that the suppression of NeuroD1 expression and the inhibition of NeuroD1/E-box interaction may play an important role in the Gc-mediated negative regulation of Pomc."xsd:string |
| http://purl.uniprot.org/citations/28406939 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0175435"xsd:string |
| http://purl.uniprot.org/citations/28406939 | http://purl.uniprot.org/core/author | "Fujiwara K."xsd:string |
| http://purl.uniprot.org/citations/28406939 | http://purl.uniprot.org/core/author | "Shimizu K."xsd:string |
| http://purl.uniprot.org/citations/28406939 | http://purl.uniprot.org/core/author | "Yokoyama A."xsd:string |
| http://purl.uniprot.org/citations/28406939 | http://purl.uniprot.org/core/author | "Shimada H."xsd:string |
| http://purl.uniprot.org/citations/28406939 | http://purl.uniprot.org/core/author | "Iwasaki Y."xsd:string |
| http://purl.uniprot.org/citations/28406939 | http://purl.uniprot.org/core/author | "Noro E."xsd:string |
| http://purl.uniprot.org/citations/28406939 | http://purl.uniprot.org/core/author | "Sugawara A."xsd:string |
| http://purl.uniprot.org/citations/28406939 | http://purl.uniprot.org/core/author | "Parvin R."xsd:string |
| http://purl.uniprot.org/citations/28406939 | http://purl.uniprot.org/core/author | "Saito-Hakoda A."xsd:string |
| http://purl.uniprot.org/citations/28406939 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28406939 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/28406939 | http://purl.uniprot.org/core/pages | "e0175435"xsd:string |
| http://purl.uniprot.org/citations/28406939 | http://purl.uniprot.org/core/title | "Role of NeuroD1 on the negative regulation of Pomc expression by glucocorticoid."xsd:string |
| http://purl.uniprot.org/citations/28406939 | http://purl.uniprot.org/core/volume | "12"xsd:string |
| http://purl.uniprot.org/citations/28406939 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28406939 |
| http://purl.uniprot.org/citations/28406939 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/28406939 |
| http://purl.uniprot.org/uniprot/#_A0A1W2P7R2-mappedCitation-28406939 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28406939 |
| http://purl.uniprot.org/uniprot/#_A0A1W2P724-mappedCitation-28406939 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28406939 |
| http://purl.uniprot.org/uniprot/#_P01193-mappedCitation-28406939 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28406939 |
| http://purl.uniprot.org/uniprot/#_Q505D0-mappedCitation-28406939 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28406939 |
| http://purl.uniprot.org/uniprot/#_Q8CEI7-mappedCitation-28406939 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28406939 |
| http://purl.uniprot.org/uniprot/#_Q60867-mappedCitation-28406939 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28406939 |