| http://purl.uniprot.org/citations/28410370 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28410370 | http://www.w3.org/2000/01/rdf-schema#comment | "In non-motile fungi, sexual reproduction relies on strong morphogenetic changes in response to pheromone signaling. We report here on a systematic screen for morphological abnormalities of the mating process in fission yeast Schizosaccharomyces pombe. We derived a homothallic (self-fertile) collection of viable deletions, which, upon visual screening, revealed a plethora of phenotypes affecting all stages of the mating process, including cell polarization, cell fusion and sporulation. Cell fusion relies on the formation of the fusion focus, an aster-like F-actin structure that is marked by strong local accumulation of the myosin V Myo52, which concentrates secretion at the fusion site. A secondary screen for fusion-defective mutants identified the myosin V Myo51-associated coiled-coil proteins Rng8 and Rng9 as critical for the coalescence of the fusion focus. Indeed, rng8Δ and rng9Δ mutant cells exhibit multiple stable dots at the cell-cell contact site, instead of the single focus observed in wildtype. Rng8 and Rng9 accumulate on the fusion focus, dependent on Myo51 and tropomyosin Cdc8. A tropomyosin mutant allele, which compromises Rng8/9 localization but not actin binding, similarly leads to multiple stable dots instead of a single focus. By contrast, myo51 deletion does not strongly affect fusion focus coalescence. We propose that focusing of the actin filaments in the fusion aster primarily relies on Rng8/9-dependent cross-linking of tropomyosin-actin filaments."xsd:string |
| http://purl.uniprot.org/citations/28410370 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pgen.1006721"xsd:string |
| http://purl.uniprot.org/citations/28410370 | http://purl.uniprot.org/core/author | "Vincenzetti V."xsd:string |
| http://purl.uniprot.org/citations/28410370 | http://purl.uniprot.org/core/author | "Martin S.G."xsd:string |
| http://purl.uniprot.org/citations/28410370 | http://purl.uniprot.org/core/author | "Bendezu F.O."xsd:string |
| http://purl.uniprot.org/citations/28410370 | http://purl.uniprot.org/core/author | "Merlini L."xsd:string |
| http://purl.uniprot.org/citations/28410370 | http://purl.uniprot.org/core/author | "Groux R."xsd:string |
| http://purl.uniprot.org/citations/28410370 | http://purl.uniprot.org/core/author | "Dudin O."xsd:string |
| http://purl.uniprot.org/citations/28410370 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28410370 | http://purl.uniprot.org/core/name | "PLoS Genet"xsd:string |
| http://purl.uniprot.org/citations/28410370 | http://purl.uniprot.org/core/pages | "e1006721"xsd:string |
| http://purl.uniprot.org/citations/28410370 | http://purl.uniprot.org/core/title | "A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion."xsd:string |
| http://purl.uniprot.org/citations/28410370 | http://purl.uniprot.org/core/volume | "13"xsd:string |
| http://purl.uniprot.org/citations/28410370 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28410370 |
| http://purl.uniprot.org/citations/28410370 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/28410370 |
| http://purl.uniprot.org/uniprot/Q9HGP5#attribution-4A64DE243ACDA7CBAB767F932333E844 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/28410370 |
| http://purl.uniprot.org/uniprot/Q9HDY9#attribution-4A64DE243ACDA7CBAB767F932333E844 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/28410370 |
| http://purl.uniprot.org/uniprot/Q10221#attribution-4A64DE243ACDA7CBAB767F932333E844 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/28410370 |
| http://purl.uniprot.org/uniprot/O94447#attribution-4A64DE243ACDA7CBAB767F932333E844 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/28410370 |
| http://purl.uniprot.org/uniprot/O94447#attribution-8B090C9AF649ADDBC80B2A6329B4808D | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/28410370 |
| http://purl.uniprot.org/uniprot/O94399#attribution-4A64DE243ACDA7CBAB767F932333E844 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/28410370 |
| http://purl.uniprot.org/uniprot/O94399#attribution-8B090C9AF649ADDBC80B2A6329B4808D | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/28410370 |
| http://purl.uniprot.org/uniprot/O94252#attribution-4A64DE243ACDA7CBAB767F932333E844 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/28410370 |
| http://purl.uniprot.org/uniprot/O94252#attribution-8B090C9AF649ADDBC80B2A6329B4808D | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/28410370 |