| http://purl.uniprot.org/citations/28413876 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28413876 | http://www.w3.org/2000/01/rdf-schema#comment | "We have engineered the protein interface of the Escherichia coli chorismate mutase (EcCM) homodimer to be dependent on incorporation of a noncanonical amino acid (ncAA) at residue 72. The large hydrophobic amino acid p-benzoyl phenylalanine (pBzF) was substituted for Tyr72, which led to a catalytically inactive protein. A library of five residues (Leu25', Arg29', Leu76, Ile80' and Asp83') surrounding pBzF72 was generated and subjected to a growth based selection in a chorismate mutase deficient strain. An EcCM variant (Phe25', pBzF72, Thr76, Gly80' and Tyr83') forms a stable homodimer, has catalytic activity similar to the wild type enzyme, and unfolds with a Tm of 53 °C. The X-ray crystal structure reveals a pi-pi stacking and hydrogen bonding interactions that stabilize the new protein interface. The strategy described here should be useful for generating organisms that are dependent on the presence of a ncAA for growth."xsd:string |
| http://purl.uniprot.org/citations/28413876 | http://purl.org/dc/terms/identifier | "doi:10.1021/jacs.7b02273"xsd:string |
| http://purl.uniprot.org/citations/28413876 | http://purl.uniprot.org/core/author | "Han G.W."xsd:string |
| http://purl.uniprot.org/citations/28413876 | http://purl.uniprot.org/core/author | "Schultz P.G."xsd:string |
| http://purl.uniprot.org/citations/28413876 | http://purl.uniprot.org/core/author | "Stevens R.C."xsd:string |
| http://purl.uniprot.org/citations/28413876 | http://purl.uniprot.org/core/author | "Nasertorabi F."xsd:string |
| http://purl.uniprot.org/citations/28413876 | http://purl.uniprot.org/core/author | "Koh M."xsd:string |
| http://purl.uniprot.org/citations/28413876 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28413876 | http://purl.uniprot.org/core/name | "J Am Chem Soc"xsd:string |
| http://purl.uniprot.org/citations/28413876 | http://purl.uniprot.org/core/pages | "5728-5731"xsd:string |
| http://purl.uniprot.org/citations/28413876 | http://purl.uniprot.org/core/title | "Generation of an Orthogonal Protein-Protein Interface with a Noncanonical Amino Acid."xsd:string |
| http://purl.uniprot.org/citations/28413876 | http://purl.uniprot.org/core/volume | "139"xsd:string |
| http://purl.uniprot.org/citations/28413876 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28413876 |
| http://purl.uniprot.org/citations/28413876 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/28413876 |
| http://purl.uniprot.org/uniprot/#_P0A9J8-mappedCitation-28413876 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28413876 |
| http://purl.uniprot.org/uniprot/P0A9J8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/28413876 |