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http://purl.uniprot.org/citations/28453731http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/28453731http://www.w3.org/2000/01/rdf-schema#comment"

Aims

Vessel maturation involves the recruitment of mural cells such as pericytes and smooth muscle cells. Laminar shear stress is a major trigger for vessel maturation, but the molecular mechanisms by which shear stress affects recruitment of pericytes are unclear. MicroRNAs (miRs) are small non-coding RNAs, which post-transcriptionally control gene expression. The aim of the present study was to unveil the mechanism by which shear stress-regulated microRNAs contribute to vessel maturation.

Methods and results

Here, we show that laminar shear stress increased miR-27a and miR-27b expression in vitro and in ex vivo in mouse femoral artery explants. Overexpression of miR-27b in endothelial cells increased pericyte adhesion and pericyte recruitment in vitro. In vitro barrier function of endothelial-pericyte co-cultures was augmented by miR-27b overexpression, whereas inhibition of miR-27a/b reduced adhesion and pericyte coverage and decreased barrier functions. In vivo, pharmacological inhibition of miR-27a/b by locked nucleic acid antisense oligonucleotides significantly reduced pericyte coverage and increased water content in the murine uterus. MiR-27b overexpression repressed semaphorins (SEMA), which mediate repulsive signals, and the vessel destabilizing human but not mouse Angiopoietin-2 (Ang-2). Silencing of SEMA6A and SEMA6D rescued the reduced pericyte adhesion by miR-27 inhibition. Furthermore, inhibition of SEMA6D increased barrier function of an endothelial-pericyte co-culture in vitro.

Conclusion

The present study demonstrates for the first time that shear stress-regulated miR-27b promotes the interaction of endothelial cells with pericytes, partly by repressing SEMA6A and SEMA6D."xsd:string
http://purl.uniprot.org/citations/28453731http://purl.org/dc/terms/identifier"doi:10.1093/cvr/cvx032"xsd:string
http://purl.uniprot.org/citations/28453731http://purl.uniprot.org/core/author"Hecker M."xsd:string
http://purl.uniprot.org/citations/28453731http://purl.uniprot.org/core/author"Liebner S."xsd:string
http://purl.uniprot.org/citations/28453731http://purl.uniprot.org/core/author"Stark K."xsd:string
http://purl.uniprot.org/citations/28453731http://purl.uniprot.org/core/author"Dimmeler S."xsd:string
http://purl.uniprot.org/citations/28453731http://purl.uniprot.org/core/author"Massberg S."xsd:string
http://purl.uniprot.org/citations/28453731http://purl.uniprot.org/core/author"Lucas T."xsd:string
http://purl.uniprot.org/citations/28453731http://purl.uniprot.org/core/author"Korff T."xsd:string
http://purl.uniprot.org/citations/28453731http://purl.uniprot.org/core/author"Doddaballapur A."xsd:string
http://purl.uniprot.org/citations/28453731http://purl.uniprot.org/core/author"Kaluza D."xsd:string
http://purl.uniprot.org/citations/28453731http://purl.uniprot.org/core/author"Zehendner C.M."xsd:string
http://purl.uniprot.org/citations/28453731http://purl.uniprot.org/core/author"Devraj K."xsd:string
http://purl.uniprot.org/citations/28453731http://purl.uniprot.org/core/author"Eckart A."xsd:string
http://purl.uniprot.org/citations/28453731http://purl.uniprot.org/core/author"Boon R.A."xsd:string
http://purl.uniprot.org/citations/28453731http://purl.uniprot.org/core/author"Manavski Y."xsd:string
http://purl.uniprot.org/citations/28453731http://purl.uniprot.org/core/author"Demolli S."xsd:string
http://purl.uniprot.org/citations/28453731http://purl.uniprot.org/core/date"2017"xsd:gYear
http://purl.uniprot.org/citations/28453731http://purl.uniprot.org/core/name"Cardiovasc Res"xsd:string
http://purl.uniprot.org/citations/28453731http://purl.uniprot.org/core/pages"681-691"xsd:string
http://purl.uniprot.org/citations/28453731http://purl.uniprot.org/core/title"Shear stress-regulated miR-27b controls pericyte recruitment by repressing SEMA6A and SEMA6D."xsd:string
http://purl.uniprot.org/citations/28453731http://purl.uniprot.org/core/volume"113"xsd:string
http://purl.uniprot.org/citations/28453731http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/28453731
http://purl.uniprot.org/citations/28453731http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/28453731
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