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http://purl.uniprot.org/citations/28457321http://www.w3.org/2000/01/rdf-schema#comment"

Background

Vascular endothelial growth factor (VEGF) and its receptors (VEGFR-1 and VEGFR-2) regulate vascular permeability and endothelial cell survival. We hypothesized that hemorrhagic shock (HS) and chronic stress (CS) would increase expression of lung VEGF and its receptors, potentiating pulmonary edema in lung tissue.

Materials and methods

Male Sprague-Dawley rats aged 8-9 wk were randomized: naïve control, lung contusion (LC), LC followed by HS (LCHS), and LCHS with CS in a restraint cylinder for 2 h/d (LCHS/CS). Animals were sacrificed on days 1 and 7. Expressions of lung VEGF, VEGFR-1, and VEGFR-2 were determined by polymerase chain reaction. Lung Injury Score (LIS) was graded on light microscopy by inflammatory cell counts, interstitial edema, pulmonary edema, and alveolar integrity (range: 0 = normal; 8 = severe injury).

Results

Seven days after LC, lung VEGF and VEGFR-1 were increased, and lung tissue healed (LIS: 0.8 ± 0.8). However, 7 d after LCHS and LCHS/CS, lung VEGF and VEGFR-1 expressions were decreased. VEGFR-2 was also decreased after LCHS/CS. LIS was elevated 7 d after LCHS and LCHS/CS (6.5 ± 1.0 and 8.2 ± 0.8). Increased LIS after LCHS and LCHS/CS was because of higher inflammatory cell counts, increased interstitial edema, and loss of alveolar integrity, whereas pulmonary edema was unchanged.

Conclusions

Elevation of lung VEGF and VEGFR-1 expressions after LC alone was associated with healing of injured lung tissue. Expressions of VEGF, VEGFR-1, and VEGFR-2 were reduced after LCHS and LCHS/CS, and injured lung tissue did not heal. Persistent lung injury after severe trauma was because of inflammation rather than pulmonary edema."xsd:string
http://purl.uniprot.org/citations/28457321http://purl.org/dc/terms/identifier"doi:10.1016/j.jss.2016.10.023"xsd:string
http://purl.uniprot.org/citations/28457321http://purl.uniprot.org/core/author"Thomson A.J."xsd:string
http://purl.uniprot.org/citations/28457321http://purl.uniprot.org/core/author"Kannan K.B."xsd:string
http://purl.uniprot.org/citations/28457321http://purl.uniprot.org/core/author"Mohr A.M."xsd:string
http://purl.uniprot.org/citations/28457321http://purl.uniprot.org/core/author"Efron P.A."xsd:string
http://purl.uniprot.org/citations/28457321http://purl.uniprot.org/core/author"Alamo I.G."xsd:string
http://purl.uniprot.org/citations/28457321http://purl.uniprot.org/core/author"Loftus T.J."xsd:string
http://purl.uniprot.org/citations/28457321http://purl.uniprot.org/core/author"Ramos H.N."xsd:string
http://purl.uniprot.org/citations/28457321http://purl.uniprot.org/core/author"Whitley E.E."xsd:string
http://purl.uniprot.org/citations/28457321http://purl.uniprot.org/core/date"2017"xsd:gYear
http://purl.uniprot.org/citations/28457321http://purl.uniprot.org/core/name"J Surg Res"xsd:string
http://purl.uniprot.org/citations/28457321http://purl.uniprot.org/core/pages"15-21"xsd:string
http://purl.uniprot.org/citations/28457321http://purl.uniprot.org/core/title"Effects of trauma, hemorrhagic shock, and chronic stress on lung vascular endothelial growth factor."xsd:string
http://purl.uniprot.org/citations/28457321http://purl.uniprot.org/core/volume"210"xsd:string
http://purl.uniprot.org/citations/28457321http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/28457321
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