| http://purl.uniprot.org/citations/28460288 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28460288 | http://www.w3.org/2000/01/rdf-schema#comment | "Platelet activation and endothelial damage play essential roles in atherosclerosis. The pathophysiology of this process is mediated by chemokines and exosomes, two critical players in cell communication. Thrombin-activated platelet-derived exosomes have protective effects on atherosclerosis and endothelial inflammation. To confirm these findings, we isolated exosomes using differential ultracentrifugation and transmission electron microscopy. The exosomes were identified using NanoSight-tracking analysis. Immunofluorescence staining and western blotting were performed to assess exosome uptake and intercellular adhesion molecule-1 (ICAM-1) expression in human umbilical vein endothelial cells (HUVECs). We found that the levels of miR-223, miR-339 and miR-21 were elevated in thrombin-activated platelet exosomes. This finding was verified in our atherosclerosis mouse model. We also found that miR-223 transfection in HUVECs inhibited ICAM-1 expression under TNF-α stimulation. Furthermore, the miR-223 inhibitor blocked the downregulating effects of exosomes on ICAM-1 expression. We examined the key proteins of two classical signaling pathways, MAPK and NF-κB, and found that miR-223 inhibited the phosphorylation of p38, JNK and ERK and blocked the nuclear translocation of NF-κB p65. Our results suggest that thrombin-activated platelet-derived exosomes inhibit ICAM-1 expression during inflammation. MiR-223 may mediate this process via regulation of the NF-κB and MAPK pathways."xsd:string |
| http://purl.uniprot.org/citations/28460288 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.thromres.2017.04.016"xsd:string |
| http://purl.uniprot.org/citations/28460288 | http://purl.uniprot.org/core/author | "Li J."xsd:string |
| http://purl.uniprot.org/citations/28460288 | http://purl.uniprot.org/core/author | "Tan M."xsd:string |
| http://purl.uniprot.org/citations/28460288 | http://purl.uniprot.org/core/author | "Xiang Q."xsd:string |
| http://purl.uniprot.org/citations/28460288 | http://purl.uniprot.org/core/author | "Zhou Z."xsd:string |
| http://purl.uniprot.org/citations/28460288 | http://purl.uniprot.org/core/author | "Yan H."xsd:string |
| http://purl.uniprot.org/citations/28460288 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28460288 | http://purl.uniprot.org/core/name | "Thromb Res"xsd:string |
| http://purl.uniprot.org/citations/28460288 | http://purl.uniprot.org/core/pages | "96-105"xsd:string |
| http://purl.uniprot.org/citations/28460288 | http://purl.uniprot.org/core/title | "Thrombin-activated platelet-derived exosomes regulate endothelial cell expression of ICAM-1 via microRNA-223 during the thrombosis-inflammation response."xsd:string |
| http://purl.uniprot.org/citations/28460288 | http://purl.uniprot.org/core/volume | "154"xsd:string |
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