| http://purl.uniprot.org/citations/28465528 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28465528 | http://www.w3.org/2000/01/rdf-schema#comment | "Wnt5a can activate Rho GTPases in chronic lymphocytic leukemia (CLL) cells by inducing the recruitment of ARHGEF2 to ROR1. Mass spectrometry on immune precipitates of Wnt5a-activated ROR1 identified 14-3-3ζ, which was confirmed by co-immunoprecipitation. The capacity of Wnt5a to induce ROR1 to complex with 14-3-3ζ could be blocked in CLL cells by treatment with cirmtuzumab, a humanized mAb targeting ROR1. Silencing 14-3-3ζ via small interfering RNA impaired the capacity of Wnt5a to: (1) induce recruitment of ARHGEF2 to ROR1, (2) enhance in vitro exchange activity of ARHGEF2 and (3) induce activation of RhoA and Rac1 in CLL cells. Furthermore, CRISPR/Cas9 deletion of 14-3-3ζ in ROR1-negative CLL cell-line MEC1, and in MEC1 cells transfected to express ROR1 (MEC1-ROR1), demonstrated that 14-3-3ζ was necessary for the growth/engraftment advantage of MEC1-ROR1 over MEC1 cells. We identified a binding motif (RSPS857SAS) in ROR1 for 14-3-3ζ. Site-directed mutagenesis of ROR1 demonstrated that serine-857 was required for the recruitment of 14-3-3ζ and ARHGEF2 to ROR1, and activation of RhoA and Rac1. Collectively, this study reveals that 14-3-3ζ plays a critical role in Wnt5a/ROR1 signaling, leading to enhanced CLL migration and proliferation."xsd:string |
| http://purl.uniprot.org/citations/28465528 | http://purl.org/dc/terms/identifier | "doi:10.1038/leu.2017.132"xsd:string |
| http://purl.uniprot.org/citations/28465528 | http://purl.uniprot.org/core/author | "Chen L."xsd:string |
| http://purl.uniprot.org/citations/28465528 | http://purl.uniprot.org/core/author | "Chen Y."xsd:string |
| http://purl.uniprot.org/citations/28465528 | http://purl.uniprot.org/core/author | "Zhang L."xsd:string |
| http://purl.uniprot.org/citations/28465528 | http://purl.uniprot.org/core/author | "Yu J."xsd:string |
| http://purl.uniprot.org/citations/28465528 | http://purl.uniprot.org/core/author | "Wu R."xsd:string |
| http://purl.uniprot.org/citations/28465528 | http://purl.uniprot.org/core/author | "Briggs S.P."xsd:string |
| http://purl.uniprot.org/citations/28465528 | http://purl.uniprot.org/core/author | "Shen Z."xsd:string |
| http://purl.uniprot.org/citations/28465528 | http://purl.uniprot.org/core/author | "Hasan M.K."xsd:string |
| http://purl.uniprot.org/citations/28465528 | http://purl.uniprot.org/core/author | "Kipps T.J."xsd:string |
| http://purl.uniprot.org/citations/28465528 | http://purl.uniprot.org/core/author | "Rassenti L.Z."xsd:string |
| http://purl.uniprot.org/citations/28465528 | http://purl.uniprot.org/core/author | "Widhopf G.F."xsd:string |
| http://purl.uniprot.org/citations/28465528 | http://purl.uniprot.org/core/author | "Ghia E.M."xsd:string |
| http://purl.uniprot.org/citations/28465528 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28465528 | http://purl.uniprot.org/core/name | "Leukemia"xsd:string |
| http://purl.uniprot.org/citations/28465528 | http://purl.uniprot.org/core/pages | "2608-2614"xsd:string |
| http://purl.uniprot.org/citations/28465528 | http://purl.uniprot.org/core/title | "Wnt5a induces ROR1 to associate with 14-3-3zeta for enhanced chemotaxis and proliferation of chronic lymphocytic leukemia cells."xsd:string |
| http://purl.uniprot.org/citations/28465528 | http://purl.uniprot.org/core/volume | "31"xsd:string |
| http://purl.uniprot.org/citations/28465528 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28465528 |
| http://purl.uniprot.org/citations/28465528 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/28465528 |
| http://purl.uniprot.org/uniprot/#_A0A384N611-mappedCitation-28465528 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28465528 |
| http://purl.uniprot.org/uniprot/#_A0FKN4-mappedCitation-28465528 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28465528 |
| http://purl.uniprot.org/uniprot/#_B3KQX9-mappedCitation-28465528 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28465528 |