| http://purl.uniprot.org/citations/28468943 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28468943 | http://www.w3.org/2000/01/rdf-schema#comment | "Estrogen plays important roles in regulation of bone formation. Cl- channels in the ClC family are expressed in osteoblasts and are associated with bone physiology and pathology, but the relationship between Cl- channels and estrogen is not clear. In this study the action of estrogen on Cl- channels was investigated in the MC3T3-E1 osteoblast cell line. Our results show that 17β-estradiol could activate a current that reversed at a potential close to the Cl- equilibrium potential, with a sequence of anion selectivity of I- > Br- > Cl- > gluconate, and was inhibited by the Cl- channel blockers 5-nitro-2-(3-phenylpropylamino)-benzoate and 4,4'-diisothiocyano-2,2'-stilbene disulfonic acid. Knockdown of ClC-3 Cl- channel expression by a specific small interfering RNA to ClC-3 attenuated activation of the 17β-estradiol-induced Cl- current. Extracellular application of membrane-impermeable 17β-estradiol-albumin conjugates activated a similar current. The estrogen-activated Cl- current could be inhibited by the estrogen receptor (ER) antagonist fulvestrant (ICI 182780). The selective ERα agonist, but not ERβ agonist, activated a Cl- current similar to that induced by 17β-estradiol. Silencing ERα expression prevented activation of estrogen-induced currents. Immunofluorescence and coimmunoprecipitation experiments demonstrated that ClC-3 Cl- channels and ERα were colocalized and closely related in cells. Estrogen promoted translocation of ClC-3 and ERα to the cell membrane from the nucleus. In conclusion, our findings show that Cl- channels can be activated by estrogen via ERα on the cell membrane and suggest that the ClC-3 Cl- channel may be one of the targets of estrogen in the regulation of osteoblast activity."xsd:string |
| http://purl.uniprot.org/citations/28468943 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajpcell.00014.2017"xsd:string |
| http://purl.uniprot.org/citations/28468943 | http://purl.uniprot.org/core/author | "Chen L."xsd:string |
| http://purl.uniprot.org/citations/28468943 | http://purl.uniprot.org/core/author | "Deng Z."xsd:string |
| http://purl.uniprot.org/citations/28468943 | http://purl.uniprot.org/core/author | "Huang Y."xsd:string |
| http://purl.uniprot.org/citations/28468943 | http://purl.uniprot.org/core/author | "Gao H."xsd:string |
| http://purl.uniprot.org/citations/28468943 | http://purl.uniprot.org/core/author | "Li Y."xsd:string |
| http://purl.uniprot.org/citations/28468943 | http://purl.uniprot.org/core/author | "Liang X."xsd:string |
| http://purl.uniprot.org/citations/28468943 | http://purl.uniprot.org/core/author | "Yang X."xsd:string |
| http://purl.uniprot.org/citations/28468943 | http://purl.uniprot.org/core/author | "Zheng Y."xsd:string |
| http://purl.uniprot.org/citations/28468943 | http://purl.uniprot.org/core/author | "Zhang H."xsd:string |
| http://purl.uniprot.org/citations/28468943 | http://purl.uniprot.org/core/author | "Wang L."xsd:string |
| http://purl.uniprot.org/citations/28468943 | http://purl.uniprot.org/core/author | "Zhu L."xsd:string |
| http://purl.uniprot.org/citations/28468943 | http://purl.uniprot.org/core/author | "Peng S."xsd:string |
| http://purl.uniprot.org/citations/28468943 | http://purl.uniprot.org/core/author | "Tan Q."xsd:string |
| http://purl.uniprot.org/citations/28468943 | http://purl.uniprot.org/core/author | "Jacob T.J.C."xsd:string |
| http://purl.uniprot.org/citations/28468943 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28468943 | http://purl.uniprot.org/core/name | "Am J Physiol Cell Physiol"xsd:string |
| http://purl.uniprot.org/citations/28468943 | http://purl.uniprot.org/core/pages | "C162-C172"xsd:string |
| http://purl.uniprot.org/citations/28468943 | http://purl.uniprot.org/core/title | "Estradiol activates chloride channels via estrogen receptor-alpha in the cell membranes of osteoblasts."xsd:string |
| http://purl.uniprot.org/citations/28468943 | http://purl.uniprot.org/core/volume | "313"xsd:string |
| http://purl.uniprot.org/citations/28468943 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28468943 |
| http://purl.uniprot.org/citations/28468943 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/28468943 |
| http://purl.uniprot.org/uniprot/#_A0A1W7GYY3-mappedCitation-28468943 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28468943 |