| http://purl.uniprot.org/citations/28475610 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28475610 | http://www.w3.org/2000/01/rdf-schema#comment | "LacZ is widely used as a reporter in studies of gene expression patterns. β-galactosidase, the product of LacZ gene, is usually detected by X-gal/FeCN staining. In X-gal/FeCN staining, β-galactosidase catalyzes X-gal to produce blue precipitates, which indicate the expression patterns of the gene of interest. A newer LacZ detection method using S-gal/TNBT is more sensitive but plagued by high background. Here, we describe an improved procedure that combines advantageous steps from the two methods. By comparing with X-gal/FeCN and S-gal/TNBT methods in detecting the expression patterns of miR-322/503 and miR-451 at a series of developmental stages, the improved method showed higher sensitivity and lower background. Thus, the improved method could be an alternative way of β-galactosidase staining in low gene expression situations."xsd:string |
| http://purl.uniprot.org/citations/28475610 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0176915"xsd:string |
| http://purl.uniprot.org/citations/28475610 | http://purl.uniprot.org/core/author | "Liu Y."xsd:string |
| http://purl.uniprot.org/citations/28475610 | http://purl.uniprot.org/core/author | "Yu W."xsd:string |
| http://purl.uniprot.org/citations/28475610 | http://purl.uniprot.org/core/author | "Shen X."xsd:string |
| http://purl.uniprot.org/citations/28475610 | http://purl.uniprot.org/core/author | "Bao W."xsd:string |
| http://purl.uniprot.org/citations/28475610 | http://purl.uniprot.org/core/author | "Liang R."xsd:string |
| http://purl.uniprot.org/citations/28475610 | http://purl.uniprot.org/core/author | "Nguyen B."xsd:string |
| http://purl.uniprot.org/citations/28475610 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28475610 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/28475610 | http://purl.uniprot.org/core/pages | "e0176915"xsd:string |
| http://purl.uniprot.org/citations/28475610 | http://purl.uniprot.org/core/title | "An improved method with high sensitivity and low background in detecting low beta-galactosidase expression in mouse embryos."xsd:string |
| http://purl.uniprot.org/citations/28475610 | http://purl.uniprot.org/core/volume | "12"xsd:string |
| http://purl.uniprot.org/citations/28475610 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28475610 |
| http://purl.uniprot.org/citations/28475610 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/28475610 |
| http://purl.uniprot.org/uniprot/#_O35474-mappedCitation-28475610 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28475610 |
| http://purl.uniprot.org/uniprot/#_Q8C4U8-mappedCitation-28475610 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28475610 |
| http://purl.uniprot.org/uniprot/#_Q8C8K0-mappedCitation-28475610 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28475610 |
| http://purl.uniprot.org/uniprot/O35474 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/28475610 |
| http://purl.uniprot.org/uniprot/Q8C4U8 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/28475610 |
| http://purl.uniprot.org/uniprot/Q8C8K0 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/28475610 |