| http://purl.uniprot.org/citations/28479320 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28479320 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28479320 | http://www.w3.org/2000/01/rdf-schema#comment | "Cytoplasmic dynein-2 powers retrograde intraflagellar transport that is essential for cilium formation and maintenance. Inactivation of dynein-2 by mutations in DYNC2H1 causes skeletal dysplasias, and it remains unclear how the dynein-2 heavy chain moves in cilia. Here, using the genome-editing technique to produce fluorescent dynein-2 heavy chain in Caenorhabditis elegans, we show by high-resolution live microscopy that dynein-2 moves in a surprising way along distinct ciliary domains. Dynein-2 shows triphasic movement in the retrograde direction: dynein-2 accelerates in the ciliary distal region and then moves at maximum velocity and finally decelerates adjacent to the base, which may represent a physical obstacle due to transition zone barriers. By knocking the conserved ciliopathy-related mutations into the C. elegans dynein-2 heavy chain, we find that these mutations reduce its transport speed and frequency. Disruption of the dynein-2 tail domain, light intermediate chain, or intraflagellar transport (IFT)-B complex abolishes dynein-2's ciliary localization, revealing their important roles in ciliary entry of dynein-2. Furthermore, our affinity purification and genetic analyses show that IFT-A subunits IFT-139 and IFT-43 function redundantly to promote dynein-2 motility. These results reveal the molecular regulation of dynein-2 movement in sensory cilia."xsd:string |
| http://purl.uniprot.org/citations/28479320 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cub.2017.04.015"xsd:string |
| http://purl.uniprot.org/citations/28479320 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cub.2017.04.015"xsd:string |
| http://purl.uniprot.org/citations/28479320 | http://purl.uniprot.org/core/author | "Dong M.Q."xsd:string |
| http://purl.uniprot.org/citations/28479320 | http://purl.uniprot.org/core/author | "Dong M.Q."xsd:string |
| http://purl.uniprot.org/citations/28479320 | http://purl.uniprot.org/core/author | "Ou G."xsd:string |
| http://purl.uniprot.org/citations/28479320 | http://purl.uniprot.org/core/author | "Ou G."xsd:string |
| http://purl.uniprot.org/citations/28479320 | http://purl.uniprot.org/core/author | "Li W.J."xsd:string |
| http://purl.uniprot.org/citations/28479320 | http://purl.uniprot.org/core/author | "Li W.J."xsd:string |
| http://purl.uniprot.org/citations/28479320 | http://purl.uniprot.org/core/author | "Yi P."xsd:string |
| http://purl.uniprot.org/citations/28479320 | http://purl.uniprot.org/core/author | "Yi P."xsd:string |
| http://purl.uniprot.org/citations/28479320 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28479320 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28479320 | http://purl.uniprot.org/core/name | "Curr. Biol."xsd:string |
| http://purl.uniprot.org/citations/28479320 | http://purl.uniprot.org/core/name | "Curr. Biol."xsd:string |
| http://purl.uniprot.org/citations/28479320 | http://purl.uniprot.org/core/pages | "1448-1461"xsd:string |
| http://purl.uniprot.org/citations/28479320 | http://purl.uniprot.org/core/pages | "1448-1461"xsd:string |
| http://purl.uniprot.org/citations/28479320 | http://purl.uniprot.org/core/title | "Dynein-driven retrograde intraflagellar transport is triphasic in C. elegans sensory cilia."xsd:string |
| http://purl.uniprot.org/citations/28479320 | http://purl.uniprot.org/core/title | "Dynein-driven retrograde intraflagellar transport is triphasic in C. elegans sensory cilia."xsd:string |
| http://purl.uniprot.org/citations/28479320 | http://purl.uniprot.org/core/volume | "27"xsd:string |
| http://purl.uniprot.org/citations/28479320 | http://purl.uniprot.org/core/volume | "27"xsd:string |
| http://purl.uniprot.org/citations/28479320 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28479320 |
| http://purl.uniprot.org/citations/28479320 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28479320 |