| http://purl.uniprot.org/citations/28497879 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28497879 | http://www.w3.org/2000/01/rdf-schema#comment | "Bone morphogenetic protein-5 (BMP-5) is a member of the TGF receptor-β family with osteoinductive property. However, its physiological role in osteoblast differentiation is not defined. This study highlights the importance of BMP-5 in MC3T3E1 osteoblast differentiation. Pre-osteoblasts exposed to osteogenic media (ascorbic acid, 50 µg/ml and β-glycerophosphate, 10 mM) showed high protein expression of BMP-5 in cell lysates and cell culture supernatants, which peaked during early time-points of differentiation and declined with onset of mineralization. Attenuation of endogenous BMP-5 protein expression by RNA interference downregulated the expression of type I collagen (COLIA1), an early osteoblast differentiation marker but not osteocalcin, a late osteoblast differentiation marker. Further experiments to analyze the cell signaling components revealed that BMP-5 modulates COLIA1 expression via p38-Runx2 axis involving Runx2 (Ser19) phosphorylation. These effects were also observed when recombinant BMP-5 was added to pre-osteoblast cultures reinforcing the fact that BMP-5 is a modulator of COLIA1 expression. We conclude that BMP-5 has stage-specific role to play during MC3T3E1 osteoblast differentiation in part by autocrine p38/Runx2/COLIA1 signaling. © 2017 BioFactors, 43(4):558-566, 2017."xsd:string |
| http://purl.uniprot.org/citations/28497879 | http://purl.org/dc/terms/identifier | "doi:10.1002/biof.1360"xsd:string |
| http://purl.uniprot.org/citations/28497879 | http://purl.uniprot.org/core/author | "Gupta S."xsd:string |
| http://purl.uniprot.org/citations/28497879 | http://purl.uniprot.org/core/author | "Vijayan V."xsd:string |
| http://purl.uniprot.org/citations/28497879 | http://purl.uniprot.org/core/author | "Gupta S.'"xsd:string |
| http://purl.uniprot.org/citations/28497879 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28497879 | http://purl.uniprot.org/core/name | "Biofactors"xsd:string |
| http://purl.uniprot.org/citations/28497879 | http://purl.uniprot.org/core/pages | "558-566"xsd:string |
| http://purl.uniprot.org/citations/28497879 | http://purl.uniprot.org/core/title | "Bone morphogenetic protein-5, a key molecule that mediates differentiation in MC3T3E1 osteoblast cell line."xsd:string |
| http://purl.uniprot.org/citations/28497879 | http://purl.uniprot.org/core/volume | "43"xsd:string |
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