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Background

Dysregulated microRNAs (miRNAs) reported to involve into the oncogenesis and progression in various human cancers. However, the roles and mechanism of miR-133 in lung adenocarcinoma remain largely unclear.

Methods

In this study, qPCR assay and western blot were used to detect the expression levels of miR-133, Akt and FLOT2. Luciferase reporter assay was used to identify the target role of miR-133 on FLOT2. The cell invasion and the migration capability were performed using the transwell invasion assay and wound healing assay.

Results

We found that miR-133 expression levels were downregulated in human lung adenocarcinoma specimens and cell lines compared with the adjacent normal tissues and normal human bronchial epithelial cell. miR-133 significantly suppressed metastasis of lung adenocarcinoma cells in vitro. Furthermore, FLOT2 (flotillin-2) identified as a direct target of miR-133, and FLOT2 expression levels were inversely correlated with miR-133 expression levels in human lung adenocarcinoma specimens. And the restoration studies suggested FGF2 as a downstream effector of miR-133 which acted through Akt signalling pathway.

Conclusions

Our study revealed the mechanism that miR-133 suppresses lung adenocarcinoma metastasis by targeting FLOT2 via Akt signalling pathway, implicating a potential prognostic biomarker and therapeutic target for lung adenocarcinoma treatment."xsd:string
http://purl.uniprot.org/citations/28503944http://purl.org/dc/terms/identifier"doi:10.1080/21691401.2017.1324467"xsd:string
http://purl.uniprot.org/citations/28503944http://purl.uniprot.org/core/author"Wei G."xsd:string
http://purl.uniprot.org/citations/28503944http://purl.uniprot.org/core/author"Xu Y."xsd:string
http://purl.uniprot.org/citations/28503944http://purl.uniprot.org/core/author"Yan J."xsd:string
http://purl.uniprot.org/citations/28503944http://purl.uniprot.org/core/author"Peng T."xsd:string
http://purl.uniprot.org/citations/28503944http://purl.uniprot.org/core/date"2018"xsd:gYear
http://purl.uniprot.org/citations/28503944http://purl.uniprot.org/core/name"Artif Cells Nanomed Biotechnol"xsd:string
http://purl.uniprot.org/citations/28503944http://purl.uniprot.org/core/pages"224-230"xsd:string
http://purl.uniprot.org/citations/28503944http://purl.uniprot.org/core/title"miR-133 involves in lung adenocarcinoma cell metastasis by targeting FLOT2."xsd:string
http://purl.uniprot.org/citations/28503944http://purl.uniprot.org/core/volume"46"xsd:string
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