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| http://purl.uniprot.org/citations/28530653 | http://www.w3.org/2000/01/rdf-schema#comment | "Intron removal requires assembly of the spliceosome on precursor mRNA (pre-mRNA) and extensive remodelling to form the spliceosome's catalytic centre. Here we report the cryo-electron microscopy structure of the yeast Saccharomyces cerevisiae pre-catalytic B complex spliceosome at near-atomic resolution. The mobile U2 small nuclear ribonucleoprotein particle (snRNP) associates with U4/U6.U5 tri-snRNP through the U2/U6 helix II and an interface between U4/U6 di-snRNP and the U2 snRNP SF3b-containing domain, which also transiently contacts the helicase Brr2. The 3' region of the U2 snRNP is flexibly attached to the SF3b-containing domain and protrudes over the concave surface of tri-snRNP, where the U1 snRNP may reside before its release from the pre-mRNA 5' splice site. The U6 ACAGAGA sequence forms a hairpin that weakly tethers the 5' splice site. The B complex proteins Prp38, Snu23 and Spp381 bind the Prp8 N-terminal domain and stabilize U6 ACAGAGA stem-pre-mRNA and Brr2-U4 small nuclear RNA interactions. These results provide important insights into the events leading to active site formation."xsd:string |
| http://purl.uniprot.org/citations/28530653 | http://purl.org/dc/terms/identifier | "doi:10.1038/nature22799"xsd:string |
| http://purl.uniprot.org/citations/28530653 | http://purl.uniprot.org/core/author | "Nagai K."xsd:string |
| http://purl.uniprot.org/citations/28530653 | http://purl.uniprot.org/core/author | "Lin P.C."xsd:string |
| http://purl.uniprot.org/citations/28530653 | http://purl.uniprot.org/core/author | "Plaschka C."xsd:string |
| http://purl.uniprot.org/citations/28530653 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28530653 | http://purl.uniprot.org/core/name | "Nature"xsd:string |
| http://purl.uniprot.org/citations/28530653 | http://purl.uniprot.org/core/pages | "617-621"xsd:string |
| http://purl.uniprot.org/citations/28530653 | http://purl.uniprot.org/core/title | "Structure of a pre-catalytic spliceosome."xsd:string |
| http://purl.uniprot.org/citations/28530653 | http://purl.uniprot.org/core/volume | "546"xsd:string |
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