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| http://purl.uniprot.org/citations/28575670 | http://www.w3.org/2000/01/rdf-schema#comment | "Centrosomes are non-membrane-bound compartments that nucleate microtubule arrays. They consist of nanometer-scale centrioles surrounded by a micron-scale, dynamic assembly of protein called the pericentriolar material (PCM). To study how PCM forms a spherical compartment that nucleates microtubules, we reconstituted PCM-dependent microtubule nucleation in vitro using recombinant C. elegans proteins. We found that macromolecular crowding drives assembly of the key PCM scaffold protein SPD-5 into spherical condensates that morphologically and dynamically resemble in vivo PCM. These SPD-5 condensates recruited the microtubule polymerase ZYG-9 (XMAP215 homolog) and the microtubule-stabilizing protein TPXL-1 (TPX2 homolog). Together, these three proteins concentrated tubulin ∼4-fold over background, which was sufficient to reconstitute nucleation of microtubule asters in vitro. Our results suggest that in vivo PCM is a selective phase that organizes microtubule arrays through localized concentration of tubulin by microtubule effector proteins."xsd:string |
| http://purl.uniprot.org/citations/28575670 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cell.2017.05.028"xsd:string |
| http://purl.uniprot.org/citations/28575670 | http://purl.uniprot.org/core/author | "Hyman A.A."xsd:string |
| http://purl.uniprot.org/citations/28575670 | http://purl.uniprot.org/core/author | "Woodruff J.B."xsd:string |
| http://purl.uniprot.org/citations/28575670 | http://purl.uniprot.org/core/author | "Honigmann A."xsd:string |
| http://purl.uniprot.org/citations/28575670 | http://purl.uniprot.org/core/author | "Mahamid J."xsd:string |
| http://purl.uniprot.org/citations/28575670 | http://purl.uniprot.org/core/author | "Widlund P.O."xsd:string |
| http://purl.uniprot.org/citations/28575670 | http://purl.uniprot.org/core/author | "Ferreira Gomes B."xsd:string |
| http://purl.uniprot.org/citations/28575670 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28575670 | http://purl.uniprot.org/core/name | "Cell"xsd:string |
| http://purl.uniprot.org/citations/28575670 | http://purl.uniprot.org/core/pages | "1066-1077.e10"xsd:string |
| http://purl.uniprot.org/citations/28575670 | http://purl.uniprot.org/core/title | "The Centrosome Is a Selective Condensate that Nucleates Microtubules by Concentrating Tubulin."xsd:string |
| http://purl.uniprot.org/citations/28575670 | http://purl.uniprot.org/core/volume | "169"xsd:string |
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