Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/28578349http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/28578349http://www.w3.org/2000/01/rdf-schema#comment"

Background/aims

Critical roles of phosphatase receptor type O (PTPRO) and toll-like receptor 4 (TLR4) have been implicated in inflammation. However, little is known about their functional effects on atherosclerosis (AS). We aim to study their potential function in AS.

Methods

An oxidized low-density lipoprotein (ox-LDL) induced AS model constructed with PTPRO over-expressing RAW264.7 cells and PTPRO knockout macrophages. Cell apoptosis was assayed by flow cytometry and fatty accumulation was evaluated by oil red staining. The production of ROS (reactive oxygen species), SOD (superoxide dismutase), MDA (malondialdehyde), TC (Triglyceride), and TG (total cholesterol) was evaluated. Western blot was performed to detect the expression of CD36, TLR4 and nuclear factor kB (NF-κB).

Results

PTPRO expression was promoted in a dose-dependent and time-dependent manner following ox-LDL challenging. In PTPRO-over-expressing cells, CD36 expression and the level of oil-red staining, TC and TG were increased; ROS production, MDA and level of cell apoptosis were improved, but SOD was reduced. However, in PTPRO knockout cells opposite results were found. TLR4 and NF-κB/p65 phosphorylation was significantly enhanced in PTPRO over-expressing cells, while significantly down-regulated in PTPRO knockout cells.

Conclusion

PTPRO plays ital roles in AS via promoting ox-LDL induced oxidative stress and cell apoptosis through TLR4/NF-κB pathway."xsd:string
http://purl.uniprot.org/citations/28578349http://purl.org/dc/terms/identifier"doi:10.1159/000477596"xsd:string
http://purl.uniprot.org/citations/28578349http://purl.uniprot.org/core/author"Li J."xsd:string
http://purl.uniprot.org/citations/28578349http://purl.uniprot.org/core/author"Hu J."xsd:string
http://purl.uniprot.org/citations/28578349http://purl.uniprot.org/core/author"Liang C."xsd:string
http://purl.uniprot.org/citations/28578349http://purl.uniprot.org/core/author"Zhang H."xsd:string
http://purl.uniprot.org/citations/28578349http://purl.uniprot.org/core/author"Wang X."xsd:string
http://purl.uniprot.org/citations/28578349http://purl.uniprot.org/core/author"Zhu T."xsd:string
http://purl.uniprot.org/citations/28578349http://purl.uniprot.org/core/author"Lian X."xsd:string
http://purl.uniprot.org/citations/28578349http://purl.uniprot.org/core/author"Gu N."xsd:string
http://purl.uniprot.org/citations/28578349http://purl.uniprot.org/core/date"2017"xsd:gYear
http://purl.uniprot.org/citations/28578349http://purl.uniprot.org/core/name"Cell Physiol Biochem"xsd:string
http://purl.uniprot.org/citations/28578349http://purl.uniprot.org/core/pages"495-505"xsd:string
http://purl.uniprot.org/citations/28578349http://purl.uniprot.org/core/title"PTPRO Promotes Oxidized Low-Density Lipoprotein Induced Oxidative Stress and Cell Apoptosis through Toll-Like Receptor 4/Nuclear Factor kappaB Pathway."xsd:string
http://purl.uniprot.org/citations/28578349http://purl.uniprot.org/core/volume"42"xsd:string
http://purl.uniprot.org/citations/28578349http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/28578349
http://purl.uniprot.org/citations/28578349http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/28578349
http://purl.uniprot.org/uniprot/#_A0A0N4SUY2-mappedCitation-28578349http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/28578349
http://purl.uniprot.org/uniprot/#_E9Q612-mappedCitation-28578349http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/28578349
http://purl.uniprot.org/uniprot/#_A2BHU8-mappedCitation-28578349http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/28578349
http://purl.uniprot.org/uniprot/#_E9Q4I1-mappedCitation-28578349http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/28578349
http://purl.uniprot.org/uniprot/#_E9PVJ6-mappedCitation-28578349http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/28578349
http://purl.uniprot.org/uniprot/#_L0CL36-mappedCitation-28578349http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/28578349
http://purl.uniprot.org/uniprot/#_Q64501-mappedCitation-28578349http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/28578349
▶